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Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
المؤلفون المشاركون
Lee, Jae-Dong
Huh, Jeong-Eun
Kang, Jung Won
Kim, Kun Hyung
Nam, Dongwoo
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-20
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
This study was conducted to explore the antiadipogenic effect and possible mechanism of Gambisan on 3T3-L1 cells.
For quality control, Gambisan was standardized by HPLC and the standard compounds ephedrine, epigallocatechin-3-gallate, and caffeine were screened.
Cultured 3T3-L1 cells that had been induced to differentiate were treated with various concentrations of Gambisan or its major component extracts (Ephedra intermedia Schrenk, Atractylodes lancea DC., and Thea sinensis L.) for 72 hours for MTT assay to determine cell viability or 10 days for LDH assay, triglyceride assay, DNA content measurement, Oil red O staining, RT-PCR, and western blot.
Gambisan significantly inhibited adipogenesis in 3T3-L1 cells by reducing triglyceride contents and lipid accumulation in a dose-dependent manner without obvious cytotoxicity.
Viability and DNA content in 3T3-L1 cells treated with Gambisan were significantly higher than cells treated with the major component extracts at every concentration.
The anti-adipogenic effects of Gambisan appeared to be mediated by a significant downregulation of the expression of lipoprotein lipase mRNA and PPARγ, C/EBPα, and SREBP-1 protein apart from the expression of hormone-sensitive lipase.
Gambisan could act as a possible therapeutic agent for obesity.
However, further studies including in vivo assays and clinical trials are needed to confirm the efficacy, safety and mechanisms of the antiobesity effects of Gambisan.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kang, Jung Won& Nam, Dongwoo& Kim, Kun Hyung& Huh, Jeong-Eun& Lee, Jae-Dong. 2013. Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-498141
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kang, Jung Won…[et al.]. Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-498141
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kang, Jung Won& Nam, Dongwoo& Kim, Kun Hyung& Huh, Jeong-Eun& Lee, Jae-Dong. Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-498141
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-498141
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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