Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
Joint Authors
Lee, Jae-Dong
Huh, Jeong-Eun
Kang, Jung Won
Kim, Kun Hyung
Nam, Dongwoo
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study was conducted to explore the antiadipogenic effect and possible mechanism of Gambisan on 3T3-L1 cells.
For quality control, Gambisan was standardized by HPLC and the standard compounds ephedrine, epigallocatechin-3-gallate, and caffeine were screened.
Cultured 3T3-L1 cells that had been induced to differentiate were treated with various concentrations of Gambisan or its major component extracts (Ephedra intermedia Schrenk, Atractylodes lancea DC., and Thea sinensis L.) for 72 hours for MTT assay to determine cell viability or 10 days for LDH assay, triglyceride assay, DNA content measurement, Oil red O staining, RT-PCR, and western blot.
Gambisan significantly inhibited adipogenesis in 3T3-L1 cells by reducing triglyceride contents and lipid accumulation in a dose-dependent manner without obvious cytotoxicity.
Viability and DNA content in 3T3-L1 cells treated with Gambisan were significantly higher than cells treated with the major component extracts at every concentration.
The anti-adipogenic effects of Gambisan appeared to be mediated by a significant downregulation of the expression of lipoprotein lipase mRNA and PPARγ, C/EBPα, and SREBP-1 protein apart from the expression of hormone-sensitive lipase.
Gambisan could act as a possible therapeutic agent for obesity.
However, further studies including in vivo assays and clinical trials are needed to confirm the efficacy, safety and mechanisms of the antiobesity effects of Gambisan.
American Psychological Association (APA)
Kang, Jung Won& Nam, Dongwoo& Kim, Kun Hyung& Huh, Jeong-Eun& Lee, Jae-Dong. 2013. Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-498141
Modern Language Association (MLA)
Kang, Jung Won…[et al.]. Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-498141
American Medical Association (AMA)
Kang, Jung Won& Nam, Dongwoo& Kim, Kun Hyung& Huh, Jeong-Eun& Lee, Jae-Dong. Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-498141
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498141