Major Components of Energy Drinks (Caffeine, Taurine, and Guarana)‎ Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production

المؤلفون المشاركون

Kolling, Eduardo Antônio
Antonio Behr, Guilherme
Resende Terra, Silvia
Ambrosi, Priscilla
Gelain, Daniel Pens
da Rocha, João Batista Teixeira
Rybarczyk-Filho, José Luiz
Pires, André S.
Zeidán-Chuliá, Fares
Fonseca Moreira, José Cláudio

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-22، 22ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-05-22

دولة النشر

مصر

عدد الصفحات

22

التخصصات الرئيسية

العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء

الملخص EN

Scope.

To elucidate the morphological and biochemical in vitro effects exerted by caffeine, taurine, and guarana, alone or in combination, since they are major components in energy drinks (EDs).

Methods and Results.

On human neuronal SH-SY5Y cells, caffeine (0.125–2 mg/mL), taurine (1–16 mg/mL), and guarana (3.125–50 mg/mL) showed concentration-dependent nonenzymatic antioxidant potential, decreased the basal levels of free radical generation, and reduced both superoxide dismutase (SOD) and catalase (CAT) activities, especially when combined together.

However, guarana-treated cells developed signs of neurite degeneration in the form of swellings at various segments in a beaded or pearl chain-like appearance and fragmentation of such neurites at concentrations ranging from 12.5 to 50 mg/mL.

Swellings, but not neuritic fragmentation, were detected when cells were treated with 0.5 mg/mL (or higher doses) of caffeine, concentrations that are present in EDs.

Cells treated with guarana also showed qualitative signs of apoptosis, including membrane blebbing, cell shrinkage, and cleaved caspase-3 positivity.

Flow cytometric analysis confirmed that cells treated with 12.5–50 mg/mL of guarana and its combinations with caffeine and/or taurine underwent apoptosis.

Conclusion.

Excessive removal of intracellular reactive oxygen species, to nonphysiological levels (or “antioxidative stress”), could be a cause of in vitro toxicity induced by these drugs.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Zeidán-Chuliá, Fares& Gelain, Daniel Pens& Kolling, Eduardo Antônio& Rybarczyk-Filho, José Luiz& Ambrosi, Priscilla& Resende Terra, Silvia…[et al.]. 2013. Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-22.
https://search.emarefa.net/detail/BIM-498436

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Zeidán-Chuliá, Fares…[et al.]. Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-22.
https://search.emarefa.net/detail/BIM-498436

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Zeidán-Chuliá, Fares& Gelain, Daniel Pens& Kolling, Eduardo Antônio& Rybarczyk-Filho, José Luiz& Ambrosi, Priscilla& Resende Terra, Silvia…[et al.]. Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-22.
https://search.emarefa.net/detail/BIM-498436

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-498436