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Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production
Joint Authors
Kolling, Eduardo Antônio
Antonio Behr, Guilherme
Resende Terra, Silvia
Ambrosi, Priscilla
Gelain, Daniel Pens
da Rocha, João Batista Teixeira
Rybarczyk-Filho, José Luiz
Pires, André S.
Zeidán-Chuliá, Fares
Fonseca Moreira, José Cláudio
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-22
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Scope.
To elucidate the morphological and biochemical in vitro effects exerted by caffeine, taurine, and guarana, alone or in combination, since they are major components in energy drinks (EDs).
Methods and Results.
On human neuronal SH-SY5Y cells, caffeine (0.125–2 mg/mL), taurine (1–16 mg/mL), and guarana (3.125–50 mg/mL) showed concentration-dependent nonenzymatic antioxidant potential, decreased the basal levels of free radical generation, and reduced both superoxide dismutase (SOD) and catalase (CAT) activities, especially when combined together.
However, guarana-treated cells developed signs of neurite degeneration in the form of swellings at various segments in a beaded or pearl chain-like appearance and fragmentation of such neurites at concentrations ranging from 12.5 to 50 mg/mL.
Swellings, but not neuritic fragmentation, were detected when cells were treated with 0.5 mg/mL (or higher doses) of caffeine, concentrations that are present in EDs.
Cells treated with guarana also showed qualitative signs of apoptosis, including membrane blebbing, cell shrinkage, and cleaved caspase-3 positivity.
Flow cytometric analysis confirmed that cells treated with 12.5–50 mg/mL of guarana and its combinations with caffeine and/or taurine underwent apoptosis.
Conclusion.
Excessive removal of intracellular reactive oxygen species, to nonphysiological levels (or “antioxidative stress”), could be a cause of in vitro toxicity induced by these drugs.
American Psychological Association (APA)
Zeidán-Chuliá, Fares& Gelain, Daniel Pens& Kolling, Eduardo Antônio& Rybarczyk-Filho, José Luiz& Ambrosi, Priscilla& Resende Terra, Silvia…[et al.]. 2013. Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-22.
https://search.emarefa.net/detail/BIM-498436
Modern Language Association (MLA)
Zeidán-Chuliá, Fares…[et al.]. Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-22.
https://search.emarefa.net/detail/BIM-498436
American Medical Association (AMA)
Zeidán-Chuliá, Fares& Gelain, Daniel Pens& Kolling, Eduardo Antônio& Rybarczyk-Filho, José Luiz& Ambrosi, Priscilla& Resende Terra, Silvia…[et al.]. Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-22.
https://search.emarefa.net/detail/BIM-498436
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498436