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Population-Sequencing as a Biomarker of Burkholderia mallei and Burkholderia pseudomallei Evolution through Microbial Forensic Analysis
المؤلفون المشاركون
Jakupciak, John P.
Karalus, Richard J.
Feldman, Andrew B.
Lin, Jeffrey S.
Wells, Jeffrey M.
Pawlowski, David R.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-17
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Large-scale genomics projects are identifying biomarkers to detect human disease.
B.
pseudomallei and B.
mallei are two closely related select agents that cause melioidosis and glanders.
Accurate characterization of metagenomic samples is dependent on accurate measurements of genetic variation between isolates with resolution down to strain level.
Often single biomarker sensitivity is augmented by use of multiple or panels of biomarkers.
In parallel with single biomarker validation, advances in DNA sequencing enable analysis of entire genomes in a single run: population-sequencing.
Potentially, direct sequencing could be used to analyze an entire genome to serve as the biomarker for genome identification.
However, genome variation and population diversity complicate use of direct sequencing, as well as differences caused by sample preparation protocols including sequencing artifacts and mistakes.
As part of a Department of Homeland Security program in bacterial forensics, we examined how to implement whole genome sequencing (WGS) analysis as a judicially defensible forensic method for attributing microbial sample relatedness; and also to determine the strengths and limitations of whole genome sequence analysis in a forensics context.
Herein, we demonstrate use of sequencing to provide genetic characterization of populations: direct sequencing of populations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jakupciak, John P.& Wells, Jeffrey M.& Karalus, Richard J.& Pawlowski, David R.& Lin, Jeffrey S.& Feldman, Andrew B.. 2013. Population-Sequencing as a Biomarker of Burkholderia mallei and Burkholderia pseudomallei Evolution through Microbial Forensic Analysis. Journal of Nucleic Acids،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-499128
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jakupciak, John P.…[et al.]. Population-Sequencing as a Biomarker of Burkholderia mallei and Burkholderia pseudomallei Evolution through Microbial Forensic Analysis. Journal of Nucleic Acids No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-499128
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jakupciak, John P.& Wells, Jeffrey M.& Karalus, Richard J.& Pawlowski, David R.& Lin, Jeffrey S.& Feldman, Andrew B.. Population-Sequencing as a Biomarker of Burkholderia mallei and Burkholderia pseudomallei Evolution through Microbial Forensic Analysis. Journal of Nucleic Acids. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-499128
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-499128
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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