Population-Sequencing as a Biomarker of Burkholderia mallei and Burkholderia pseudomallei Evolution through Microbial Forensic Analysis
Joint Authors
Jakupciak, John P.
Karalus, Richard J.
Feldman, Andrew B.
Lin, Jeffrey S.
Wells, Jeffrey M.
Pawlowski, David R.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-17
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Large-scale genomics projects are identifying biomarkers to detect human disease.
B.
pseudomallei and B.
mallei are two closely related select agents that cause melioidosis and glanders.
Accurate characterization of metagenomic samples is dependent on accurate measurements of genetic variation between isolates with resolution down to strain level.
Often single biomarker sensitivity is augmented by use of multiple or panels of biomarkers.
In parallel with single biomarker validation, advances in DNA sequencing enable analysis of entire genomes in a single run: population-sequencing.
Potentially, direct sequencing could be used to analyze an entire genome to serve as the biomarker for genome identification.
However, genome variation and population diversity complicate use of direct sequencing, as well as differences caused by sample preparation protocols including sequencing artifacts and mistakes.
As part of a Department of Homeland Security program in bacterial forensics, we examined how to implement whole genome sequencing (WGS) analysis as a judicially defensible forensic method for attributing microbial sample relatedness; and also to determine the strengths and limitations of whole genome sequence analysis in a forensics context.
Herein, we demonstrate use of sequencing to provide genetic characterization of populations: direct sequencing of populations.
American Psychological Association (APA)
Jakupciak, John P.& Wells, Jeffrey M.& Karalus, Richard J.& Pawlowski, David R.& Lin, Jeffrey S.& Feldman, Andrew B.. 2013. Population-Sequencing as a Biomarker of Burkholderia mallei and Burkholderia pseudomallei Evolution through Microbial Forensic Analysis. Journal of Nucleic Acids،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-499128
Modern Language Association (MLA)
Jakupciak, John P.…[et al.]. Population-Sequencing as a Biomarker of Burkholderia mallei and Burkholderia pseudomallei Evolution through Microbial Forensic Analysis. Journal of Nucleic Acids No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-499128
American Medical Association (AMA)
Jakupciak, John P.& Wells, Jeffrey M.& Karalus, Richard J.& Pawlowski, David R.& Lin, Jeffrey S.& Feldman, Andrew B.. Population-Sequencing as a Biomarker of Burkholderia mallei and Burkholderia pseudomallei Evolution through Microbial Forensic Analysis. Journal of Nucleic Acids. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-499128
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499128