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Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts
المؤلفون المشاركون
Tomaselli, Gordon F.
Weisser-Thomas, Jutta
O'Rourke, Brian
Houser, Steven R.
Ohler, Andreas
Piacentino, Valentino
المصدر
Cardiology Research and Practice
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-03-07
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objective.
The transverse-axial tubule system (TATS) of cardiomyocytes allows a spatially coordinated conversion of electrical excitation into an intracellular Ca2+ signal and consequently contraction.
Previous reports have indicated alterations of structure and/or volume of the TATS in cardiac hypertrophy and failure, suggesting a contribution to the impairment of excitation contraction coupling.
To test whether structural alterations are present in human heart failure, the TATS was visualized in myocytes from failing and non-failing human hearts.
Methods and Results.
In freshly isolated myocytes, the plasmalemmal membranes were labeled with Di-8-ANEPPS and imaged using two-photon excitation at 780 nm.
Optical sections were taken every 300 nm through the cells.
After deconvolution, the TATS was determined within the 3D data sets, revealing no significant difference in normalized surface area or volume.
To rule out possible inhomogeneity in the arrangement of the TATS, Euclidian distance maps were plotted for every section, allowing to measure the closest distance between any cytosolic and any membrane point.
There was a trend towards greater spacing in cells from failing hearts, without statistical significance.
Conclusion.
Only small changes, but no significant changes in the geometrical dimensions of the TATS were observed in cardiomyocytes from failing compared to non-failing human myocardium.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ohler, Andreas& Weisser-Thomas, Jutta& Piacentino, Valentino& Houser, Steven R.& Tomaselli, Gordon F.& O'Rourke, Brian. 2010. Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts. Cardiology Research and Practice،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-499202
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ohler, Andreas…[et al.]. Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts. Cardiology Research and Practice No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-499202
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ohler, Andreas& Weisser-Thomas, Jutta& Piacentino, Valentino& Houser, Steven R.& Tomaselli, Gordon F.& O'Rourke, Brian. Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts. Cardiology Research and Practice. 2010. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-499202
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-499202
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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