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Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts
Joint Authors
Tomaselli, Gordon F.
Weisser-Thomas, Jutta
O'Rourke, Brian
Houser, Steven R.
Ohler, Andreas
Piacentino, Valentino
Source
Cardiology Research and Practice
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
The transverse-axial tubule system (TATS) of cardiomyocytes allows a spatially coordinated conversion of electrical excitation into an intracellular Ca2+ signal and consequently contraction.
Previous reports have indicated alterations of structure and/or volume of the TATS in cardiac hypertrophy and failure, suggesting a contribution to the impairment of excitation contraction coupling.
To test whether structural alterations are present in human heart failure, the TATS was visualized in myocytes from failing and non-failing human hearts.
Methods and Results.
In freshly isolated myocytes, the plasmalemmal membranes were labeled with Di-8-ANEPPS and imaged using two-photon excitation at 780 nm.
Optical sections were taken every 300 nm through the cells.
After deconvolution, the TATS was determined within the 3D data sets, revealing no significant difference in normalized surface area or volume.
To rule out possible inhomogeneity in the arrangement of the TATS, Euclidian distance maps were plotted for every section, allowing to measure the closest distance between any cytosolic and any membrane point.
There was a trend towards greater spacing in cells from failing hearts, without statistical significance.
Conclusion.
Only small changes, but no significant changes in the geometrical dimensions of the TATS were observed in cardiomyocytes from failing compared to non-failing human myocardium.
American Psychological Association (APA)
Ohler, Andreas& Weisser-Thomas, Jutta& Piacentino, Valentino& Houser, Steven R.& Tomaselli, Gordon F.& O'Rourke, Brian. 2010. Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts. Cardiology Research and Practice،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-499202
Modern Language Association (MLA)
Ohler, Andreas…[et al.]. Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts. Cardiology Research and Practice No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-499202
American Medical Association (AMA)
Ohler, Andreas& Weisser-Thomas, Jutta& Piacentino, Valentino& Houser, Steven R.& Tomaselli, Gordon F.& O'Rourke, Brian. Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts. Cardiology Research and Practice. 2010. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-499202
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499202