Generation of Insulin-Producing Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells : Comparison of Three Differentiation Protocols
المؤلفون المشاركون
Ghoneim, Mohamed A.
Khater, Sherry M.
Ashamallah, Sylvia A.
Ismail, Amani M.
Gabr, Mahmoud M.
Zakaria, Mahmoud M.
El-Badri, Nagwa
Refaie, Ayman F.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-10
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Introduction.
Many protocols were utilized for directed differentiation of mesenchymal stem cells (MSCs) to form insulin-producing cells (IPCs).
We compared the relative efficiency of three differentiation protocols.
Methods.
Human bone marrow-derived MSCs (HBM-MSCs) were obtained from three insulin-dependent type 2 diabetic patients.
Differentiation into IPCs was carried out by three protocols: conophylline-based (one-step protocol), trichostatin-A-based (two-step protocol), and β-mercaptoethanol-based (three-step protocol).
At the end of differentiation, cells were evaluated by immunolabeling for insulin production, expression of pancreatic endocrine genes, and release of insulin and c-peptide in response to increasing glucose concentrations.
Results.
By immunolabeling, the proportion of generated IPCs was modest (≃3%) in all the three protocols.
All relevant pancreatic endocrine genes, insulin, glucagon, and somatostatin, were expressed.
There was a stepwise increase in insulin and c-peptide release in response to glucose challenge, but the released amounts were low when compared with those of pancreatic islets.
Conclusion.
The yield of functional IPCs following directed differentiation of HBM-MSCs was modest and was comparable among the three tested protocols.
Protocols for directed differentiation of MSCs need further optimization in order to be clinically meaningful.
To this end, addition of an extracellular matrix and/or a suitable template should be attempted.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gabr, Mahmoud M.& Zakaria, Mahmoud M.& Refaie, Ayman F.& Khater, Sherry M.& Ashamallah, Sylvia A.& Ismail, Amani M.…[et al.]. 2014. Generation of Insulin-Producing Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells : Comparison of Three Differentiation Protocols. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-501780
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gabr, Mahmoud M.…[et al.]. Generation of Insulin-Producing Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells : Comparison of Three Differentiation Protocols. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-501780
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gabr, Mahmoud M.& Zakaria, Mahmoud M.& Refaie, Ayman F.& Khater, Sherry M.& Ashamallah, Sylvia A.& Ismail, Amani M.…[et al.]. Generation of Insulin-Producing Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells : Comparison of Three Differentiation Protocols. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-501780
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-501780
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر