Generation of Insulin-Producing Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells : Comparison of Three Differentiation Protocols
Joint Authors
Ghoneim, Mohamed A.
Khater, Sherry M.
Ashamallah, Sylvia A.
Ismail, Amani M.
Gabr, Mahmoud M.
Zakaria, Mahmoud M.
El-Badri, Nagwa
Refaie, Ayman F.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Introduction.
Many protocols were utilized for directed differentiation of mesenchymal stem cells (MSCs) to form insulin-producing cells (IPCs).
We compared the relative efficiency of three differentiation protocols.
Methods.
Human bone marrow-derived MSCs (HBM-MSCs) were obtained from three insulin-dependent type 2 diabetic patients.
Differentiation into IPCs was carried out by three protocols: conophylline-based (one-step protocol), trichostatin-A-based (two-step protocol), and β-mercaptoethanol-based (three-step protocol).
At the end of differentiation, cells were evaluated by immunolabeling for insulin production, expression of pancreatic endocrine genes, and release of insulin and c-peptide in response to increasing glucose concentrations.
Results.
By immunolabeling, the proportion of generated IPCs was modest (≃3%) in all the three protocols.
All relevant pancreatic endocrine genes, insulin, glucagon, and somatostatin, were expressed.
There was a stepwise increase in insulin and c-peptide release in response to glucose challenge, but the released amounts were low when compared with those of pancreatic islets.
Conclusion.
The yield of functional IPCs following directed differentiation of HBM-MSCs was modest and was comparable among the three tested protocols.
Protocols for directed differentiation of MSCs need further optimization in order to be clinically meaningful.
To this end, addition of an extracellular matrix and/or a suitable template should be attempted.
American Psychological Association (APA)
Gabr, Mahmoud M.& Zakaria, Mahmoud M.& Refaie, Ayman F.& Khater, Sherry M.& Ashamallah, Sylvia A.& Ismail, Amani M.…[et al.]. 2014. Generation of Insulin-Producing Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells : Comparison of Three Differentiation Protocols. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-501780
Modern Language Association (MLA)
Gabr, Mahmoud M.…[et al.]. Generation of Insulin-Producing Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells : Comparison of Three Differentiation Protocols. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-501780
American Medical Association (AMA)
Gabr, Mahmoud M.& Zakaria, Mahmoud M.& Refaie, Ayman F.& Khater, Sherry M.& Ashamallah, Sylvia A.& Ismail, Amani M.…[et al.]. Generation of Insulin-Producing Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells : Comparison of Three Differentiation Protocols. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-501780
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-501780