Proteomic Analysis of Gossypol Induces Necrosis in Multiple Myeloma Cells
المؤلفون المشاركون
Xu, Renhua
Tang, Haiping
Wang, Qingtao
Liu, Chongdong
Tian, Enbing
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-14
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Gossypol is a phenolic aldehyde extracted from plants and is known to be an antitumor agent to induce cancer cell apoptosis.
In the present study, multiple myeloma cells were treated with gossypol, which resulted in an increase of cellular reactive oxygen species (ROS) and cell necrosis.
Quantitative proteomic analysis was carried out to identify differentially expressed proteins between untreated and gossypol-treated cells.
Proteomic analysis identified 4330 proteins, in which 202 proteins are upregulated and 383 proteins are downregulated in gossypol-treated cells as compared to the untreated cells.
Importantly, proteomic and western blot analysis showed that apoptosis regulators BAK and Bax were upregulated in gossypol-treated cells, indicating that Bcl-2 associated death pathway was activated.
Similarly, gossypol also induced upregulations of DNA mismatch repair proteins and DNA replication licensing factor, suggesting that gossypol caused significant DNA damage.
Furthermore, upregulations of HLA class I and class II histocompatibility antigens and beta-2-microglobulin were observed in gossypol-treated cells, indicating that gossypol has a novel function to activate cellular immune responses.
Our data demonstrate that the execution of necrosis is a complex process involving ROS, DNA damage, and Bcl-2 family proteins.
Gossypol-activated immune responses are a potential new approach for multiple myeloma chemotherapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Renhua& Tian, Enbing& Tang, Haiping& Liu, Chongdong& Wang, Qingtao. 2014. Proteomic Analysis of Gossypol Induces Necrosis in Multiple Myeloma Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-502272
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Renhua…[et al.]. Proteomic Analysis of Gossypol Induces Necrosis in Multiple Myeloma Cells. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-502272
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Renhua& Tian, Enbing& Tang, Haiping& Liu, Chongdong& Wang, Qingtao. Proteomic Analysis of Gossypol Induces Necrosis in Multiple Myeloma Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-502272
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-502272
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر