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Proteomic Analysis of Gossypol Induces Necrosis in Multiple Myeloma Cells
Joint Authors
Xu, Renhua
Tang, Haiping
Wang, Qingtao
Liu, Chongdong
Tian, Enbing
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Gossypol is a phenolic aldehyde extracted from plants and is known to be an antitumor agent to induce cancer cell apoptosis.
In the present study, multiple myeloma cells were treated with gossypol, which resulted in an increase of cellular reactive oxygen species (ROS) and cell necrosis.
Quantitative proteomic analysis was carried out to identify differentially expressed proteins between untreated and gossypol-treated cells.
Proteomic analysis identified 4330 proteins, in which 202 proteins are upregulated and 383 proteins are downregulated in gossypol-treated cells as compared to the untreated cells.
Importantly, proteomic and western blot analysis showed that apoptosis regulators BAK and Bax were upregulated in gossypol-treated cells, indicating that Bcl-2 associated death pathway was activated.
Similarly, gossypol also induced upregulations of DNA mismatch repair proteins and DNA replication licensing factor, suggesting that gossypol caused significant DNA damage.
Furthermore, upregulations of HLA class I and class II histocompatibility antigens and beta-2-microglobulin were observed in gossypol-treated cells, indicating that gossypol has a novel function to activate cellular immune responses.
Our data demonstrate that the execution of necrosis is a complex process involving ROS, DNA damage, and Bcl-2 family proteins.
Gossypol-activated immune responses are a potential new approach for multiple myeloma chemotherapy.
American Psychological Association (APA)
Xu, Renhua& Tian, Enbing& Tang, Haiping& Liu, Chongdong& Wang, Qingtao. 2014. Proteomic Analysis of Gossypol Induces Necrosis in Multiple Myeloma Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-502272
Modern Language Association (MLA)
Xu, Renhua…[et al.]. Proteomic Analysis of Gossypol Induces Necrosis in Multiple Myeloma Cells. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-502272
American Medical Association (AMA)
Xu, Renhua& Tian, Enbing& Tang, Haiping& Liu, Chongdong& Wang, Qingtao. Proteomic Analysis of Gossypol Induces Necrosis in Multiple Myeloma Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-502272
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-502272