Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity

المؤلفون المشاركون

Maier, Jeanette A. M.
Castiglioni, Sara
Cazzaniga, Alessandra

المصدر

BioMed Research International

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-08-19

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الطب البشري

الملخص EN

Background and Aims.

Gravity contributes to the maintenance of bone integrity.

Accordingly, weightlessness conditions during space flight accelerate bone loss and experimental models in real and simulated microgravity show decreased osteoblastic and increased osteoclastic activities.

It is well known that the endothelium and bone cells cross-talk and this intercellular communication is vital to regulate bone homeostasis.

Because microgravity promotes microvascular endothelial dysfunction, we anticipated that the molecular cross-talk between endothelial cells exposed to simulated microgravity and osteoblasts might be altered.

Results.

We cultured human microvascular endothelial cells in simulated microgravity using the rotating wall vessel device developed by NASA.

Endothelial cells in microgravity show growth inhibition and release higher amounts of matrix metalloproteases type 2 and interleukin-6 than controls.

Conditioned media collected from microvascular endothelial cells in simulated microgravity were used to culture human osteoblasts and were shown to retard osteoblast proliferation and inhibit their activity.

Discussion.

Microvascular endothelial cells in microgravity are growth retarded and release high amounts of matrix metalloproteases type 2 and interleukin-6, which might play a role in retarding the growth of osteoblasts and impairing their osteogenic activity.

Conclusions.

We demonstrate that since simulated microgravity modulates microvascular endothelial cell function, it indirectly impairs osteoblastic function.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Cazzaniga, Alessandra& Castiglioni, Sara& Maier, Jeanette A. M.. 2014. Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-503871

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Cazzaniga, Alessandra…[et al.]. Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-503871

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Cazzaniga, Alessandra& Castiglioni, Sara& Maier, Jeanette A. M.. Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-503871

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-503871