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Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity
Joint Authors
Maier, Jeanette A. M.
Castiglioni, Sara
Cazzaniga, Alessandra
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background and Aims.
Gravity contributes to the maintenance of bone integrity.
Accordingly, weightlessness conditions during space flight accelerate bone loss and experimental models in real and simulated microgravity show decreased osteoblastic and increased osteoclastic activities.
It is well known that the endothelium and bone cells cross-talk and this intercellular communication is vital to regulate bone homeostasis.
Because microgravity promotes microvascular endothelial dysfunction, we anticipated that the molecular cross-talk between endothelial cells exposed to simulated microgravity and osteoblasts might be altered.
Results.
We cultured human microvascular endothelial cells in simulated microgravity using the rotating wall vessel device developed by NASA.
Endothelial cells in microgravity show growth inhibition and release higher amounts of matrix metalloproteases type 2 and interleukin-6 than controls.
Conditioned media collected from microvascular endothelial cells in simulated microgravity were used to culture human osteoblasts and were shown to retard osteoblast proliferation and inhibit their activity.
Discussion.
Microvascular endothelial cells in microgravity are growth retarded and release high amounts of matrix metalloproteases type 2 and interleukin-6, which might play a role in retarding the growth of osteoblasts and impairing their osteogenic activity.
Conclusions.
We demonstrate that since simulated microgravity modulates microvascular endothelial cell function, it indirectly impairs osteoblastic function.
American Psychological Association (APA)
Cazzaniga, Alessandra& Castiglioni, Sara& Maier, Jeanette A. M.. 2014. Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-503871
Modern Language Association (MLA)
Cazzaniga, Alessandra…[et al.]. Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-503871
American Medical Association (AMA)
Cazzaniga, Alessandra& Castiglioni, Sara& Maier, Jeanette A. M.. Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-503871
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503871