Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR
المؤلفون المشاركون
Mahmoudi Rad, Mahnaz
Amel Zabihi, Maryam
Mirdamadi, Yasaman
Zafarghandi, Ameneh Sh
Tavallaee, Mahkam
المصدر
Infectious Diseases in Obstetrics and Gynecology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-06-26
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Background.
Vulvovaginal candidiasis is a common infection.
The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique.
Methods.
191 isolates from patients admitted to Mahdieh hospital were identified.
The vaginal swab specimens were cultured on Sabouraud Dextrose Agar.
The ITS1 region between the 18S and 5.8S rRNA genes and a specific DNA fragment within the ITS2 region were amplified.
The multiplex PCR products were separated by electrophoresis in 2% agarose gel, visualized by staining with ethidium bromide, and photographed.
Descriptive statistics, Chi-square test, and Spearman correlation were used to summarize the findings.
Results.
C.
albicans and C.
glabrata were the most common species isolated from the specimens.
A mix of C.
glabrata and C.
albicans was the most common mixed infection isolated from the samples.
The analysis revealed a significant positive association between older age and infection with C.
glabrata isolates (Spearman’s rho = 0.89, P=0.015).
Conclusion.
Multiplex PCR is a fast, yet reliable method to identify Candida species.
C.
albicans and then C.
glabrata are the two most common causes of vulvovaginal candidiasis.
The number of mixed fungal infections is higher among Iranian population compared to international reports.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mahmoudi Rad, Mahnaz& Zafarghandi, Ameneh Sh& Amel Zabihi, Maryam& Tavallaee, Mahkam& Mirdamadi, Yasaman. 2012. Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR. Infectious Diseases in Obstetrics and Gynecology،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-505085
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mahmoudi Rad, Mahnaz…[et al.]. Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR. Infectious Diseases in Obstetrics and Gynecology No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-505085
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mahmoudi Rad, Mahnaz& Zafarghandi, Ameneh Sh& Amel Zabihi, Maryam& Tavallaee, Mahkam& Mirdamadi, Yasaman. Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR. Infectious Diseases in Obstetrics and Gynecology. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-505085
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-505085
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر