Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR
Joint Authors
Mahmoudi Rad, Mahnaz
Amel Zabihi, Maryam
Mirdamadi, Yasaman
Zafarghandi, Ameneh Sh
Tavallaee, Mahkam
Source
Infectious Diseases in Obstetrics and Gynecology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-26
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Background.
Vulvovaginal candidiasis is a common infection.
The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique.
Methods.
191 isolates from patients admitted to Mahdieh hospital were identified.
The vaginal swab specimens were cultured on Sabouraud Dextrose Agar.
The ITS1 region between the 18S and 5.8S rRNA genes and a specific DNA fragment within the ITS2 region were amplified.
The multiplex PCR products were separated by electrophoresis in 2% agarose gel, visualized by staining with ethidium bromide, and photographed.
Descriptive statistics, Chi-square test, and Spearman correlation were used to summarize the findings.
Results.
C.
albicans and C.
glabrata were the most common species isolated from the specimens.
A mix of C.
glabrata and C.
albicans was the most common mixed infection isolated from the samples.
The analysis revealed a significant positive association between older age and infection with C.
glabrata isolates (Spearman’s rho = 0.89, P=0.015).
Conclusion.
Multiplex PCR is a fast, yet reliable method to identify Candida species.
C.
albicans and then C.
glabrata are the two most common causes of vulvovaginal candidiasis.
The number of mixed fungal infections is higher among Iranian population compared to international reports.
American Psychological Association (APA)
Mahmoudi Rad, Mahnaz& Zafarghandi, Ameneh Sh& Amel Zabihi, Maryam& Tavallaee, Mahkam& Mirdamadi, Yasaman. 2012. Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR. Infectious Diseases in Obstetrics and Gynecology،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-505085
Modern Language Association (MLA)
Mahmoudi Rad, Mahnaz…[et al.]. Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR. Infectious Diseases in Obstetrics and Gynecology No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-505085
American Medical Association (AMA)
Mahmoudi Rad, Mahnaz& Zafarghandi, Ameneh Sh& Amel Zabihi, Maryam& Tavallaee, Mahkam& Mirdamadi, Yasaman. Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR. Infectious Diseases in Obstetrics and Gynecology. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-505085
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505085