Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A
المؤلفون المشاركون
Leon, Deborah R.
Loo, Joseph A.
Rohlin, Lars
Ogorzalek Loo, Rachel R.
Gunsalus, Robert P.
Kim, Unmi
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-05-15
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Many archaeal cell envelopes contain a protein coat or sheath composed of one or more surface exposed proteins.
These surface layer (S-layer) proteins contribute structural integrity and protect the lipid membrane from environmental challenges.
To explore the species diversity of these layers in the Methanosarcinaceae, the major S-layer protein in Methanosarcina barkeri strain Fusaro was identified using proteomics.
The Mbar_A1758 gene product was present in multiple forms with apparent sizes of 130, 120, and 100 kDa, consistent with post-translational modifications including signal peptide excision and protein glycosylation.
A protein with features related to the surface layer proteins found in Methanosarcina acetivorans C2A and Methanosarcina mazei Goel was identified in the M.
barkeri genome.
These data reveal a distinct conserved protein signature with features and implied cell surface architecture in the Methanosarcinaceae that is absent in other archaea.
Paralogous gene expression patterns in two Methanosarcina species revealed abundant expression of a single S-layer paralog in each strain.
Respective promoter elements were identified and shown to be conserved in mRNA coding and upstream untranslated regions.
Prior M.
acetivorans genome annotations assigned S-layer or surface layer associated roles of eighty genes: however, of 68 examined none was significantly expressed relative to the experimentally determined S-layer gene.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rohlin, Lars& Leon, Deborah R.& Kim, Unmi& Loo, Joseph A.& Ogorzalek Loo, Rachel R.& Gunsalus, Robert P.. 2012. Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A. Archaea،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-505204
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rohlin, Lars…[et al.]. Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A. Archaea No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-505204
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rohlin, Lars& Leon, Deborah R.& Kim, Unmi& Loo, Joseph A.& Ogorzalek Loo, Rachel R.& Gunsalus, Robert P.. Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A. Archaea. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-505204
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-505204
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر