Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A

Joint Authors

Leon, Deborah R.
Loo, Joseph A.
Rohlin, Lars
Ogorzalek Loo, Rachel R.
Gunsalus, Robert P.
Kim, Unmi

Source

Archaea

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-05-15

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Biology

Abstract EN

Many archaeal cell envelopes contain a protein coat or sheath composed of one or more surface exposed proteins.

These surface layer (S-layer) proteins contribute structural integrity and protect the lipid membrane from environmental challenges.

To explore the species diversity of these layers in the Methanosarcinaceae, the major S-layer protein in Methanosarcina barkeri strain Fusaro was identified using proteomics.

The Mbar_A1758 gene product was present in multiple forms with apparent sizes of 130, 120, and 100 kDa, consistent with post-translational modifications including signal peptide excision and protein glycosylation.

A protein with features related to the surface layer proteins found in Methanosarcina acetivorans C2A and Methanosarcina mazei Goel was identified in the M.

barkeri genome.

These data reveal a distinct conserved protein signature with features and implied cell surface architecture in the Methanosarcinaceae that is absent in other archaea.

Paralogous gene expression patterns in two Methanosarcina species revealed abundant expression of a single S-layer paralog in each strain.

Respective promoter elements were identified and shown to be conserved in mRNA coding and upstream untranslated regions.

Prior M.

acetivorans genome annotations assigned S-layer or surface layer associated roles of eighty genes: however, of 68 examined none was significantly expressed relative to the experimentally determined S-layer gene.

American Psychological Association (APA)

Rohlin, Lars& Leon, Deborah R.& Kim, Unmi& Loo, Joseph A.& Ogorzalek Loo, Rachel R.& Gunsalus, Robert P.. 2012. Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A. Archaea،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-505204

Modern Language Association (MLA)

Rohlin, Lars…[et al.]. Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A. Archaea No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-505204

American Medical Association (AMA)

Rohlin, Lars& Leon, Deborah R.& Kim, Unmi& Loo, Joseph A.& Ogorzalek Loo, Rachel R.& Gunsalus, Robert P.. Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A. Archaea. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-505204

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-505204