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Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A
Joint Authors
Leon, Deborah R.
Loo, Joseph A.
Rohlin, Lars
Ogorzalek Loo, Rachel R.
Gunsalus, Robert P.
Kim, Unmi
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Many archaeal cell envelopes contain a protein coat or sheath composed of one or more surface exposed proteins.
These surface layer (S-layer) proteins contribute structural integrity and protect the lipid membrane from environmental challenges.
To explore the species diversity of these layers in the Methanosarcinaceae, the major S-layer protein in Methanosarcina barkeri strain Fusaro was identified using proteomics.
The Mbar_A1758 gene product was present in multiple forms with apparent sizes of 130, 120, and 100 kDa, consistent with post-translational modifications including signal peptide excision and protein glycosylation.
A protein with features related to the surface layer proteins found in Methanosarcina acetivorans C2A and Methanosarcina mazei Goel was identified in the M.
barkeri genome.
These data reveal a distinct conserved protein signature with features and implied cell surface architecture in the Methanosarcinaceae that is absent in other archaea.
Paralogous gene expression patterns in two Methanosarcina species revealed abundant expression of a single S-layer paralog in each strain.
Respective promoter elements were identified and shown to be conserved in mRNA coding and upstream untranslated regions.
Prior M.
acetivorans genome annotations assigned S-layer or surface layer associated roles of eighty genes: however, of 68 examined none was significantly expressed relative to the experimentally determined S-layer gene.
American Psychological Association (APA)
Rohlin, Lars& Leon, Deborah R.& Kim, Unmi& Loo, Joseph A.& Ogorzalek Loo, Rachel R.& Gunsalus, Robert P.. 2012. Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A. Archaea،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-505204
Modern Language Association (MLA)
Rohlin, Lars…[et al.]. Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A. Archaea No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-505204
American Medical Association (AMA)
Rohlin, Lars& Leon, Deborah R.& Kim, Unmi& Loo, Joseph A.& Ogorzalek Loo, Rachel R.& Gunsalus, Robert P.. Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea : Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A. Archaea. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-505204
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505204