The Effect of Hypoxia on the Stemness and Differentiation Capacity of PDLC and DPC
المؤلفون المشاركون
Zhou, Yinghong
Fan, Wei
Xiao, Yin
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-20
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Introduction.
Stem cells are regularly cultured under normoxic conditions.
However, the physiological oxygen tension in the stem cell niche is known to be as low as 1-2% oxygen, suggesting that hypoxia has a distinct impact on stem cell maintenance.
Periodontal ligament cells (PDLCs) and dental pulp cells (DPCs) are attractive candidates in dental tissue regeneration.
It is of great interest to know whether hypoxia plays a role in maintaining the stemness and differentiation capacity of PDLCs and DPCs.
Methods.
PDLCs and DPCs were cultured either in normoxia (20% O2) or hypoxia (2% O2).
Cell viability assays were performed and the expressions of pluripotency markers (Oct-4, Sox2, and c-Myc) were detected by qRT-PCR and western blotting.
Mineralization, glycosaminoglycan (GAG) deposition, and lipid droplets formation were assessed by Alizarin red S, Safranin O, and Oil red O staining, respectively.
Results.
Hypoxia did not show negative effects on the proliferation of PDLCs and DPCs.
The pluripotency markers and differentiation potentials of PDLCs and DPCs significantly increased in response to hypoxic environment.
Conclusions.
Our findings suggest that hypoxia plays an important role in maintaining the stemness and differentiation capacity of PDLCs and DPCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Yinghong& Fan, Wei& Xiao, Yin. 2014. The Effect of Hypoxia on the Stemness and Differentiation Capacity of PDLC and DPC. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-505752
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Yinghong…[et al.]. The Effect of Hypoxia on the Stemness and Differentiation Capacity of PDLC and DPC. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-505752
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Yinghong& Fan, Wei& Xiao, Yin. The Effect of Hypoxia on the Stemness and Differentiation Capacity of PDLC and DPC. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-505752
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-505752
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر