The Effect of Hypoxia on the Stemness and Differentiation Capacity of PDLC and DPC
Joint Authors
Zhou, Yinghong
Fan, Wei
Xiao, Yin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Introduction.
Stem cells are regularly cultured under normoxic conditions.
However, the physiological oxygen tension in the stem cell niche is known to be as low as 1-2% oxygen, suggesting that hypoxia has a distinct impact on stem cell maintenance.
Periodontal ligament cells (PDLCs) and dental pulp cells (DPCs) are attractive candidates in dental tissue regeneration.
It is of great interest to know whether hypoxia plays a role in maintaining the stemness and differentiation capacity of PDLCs and DPCs.
Methods.
PDLCs and DPCs were cultured either in normoxia (20% O2) or hypoxia (2% O2).
Cell viability assays were performed and the expressions of pluripotency markers (Oct-4, Sox2, and c-Myc) were detected by qRT-PCR and western blotting.
Mineralization, glycosaminoglycan (GAG) deposition, and lipid droplets formation were assessed by Alizarin red S, Safranin O, and Oil red O staining, respectively.
Results.
Hypoxia did not show negative effects on the proliferation of PDLCs and DPCs.
The pluripotency markers and differentiation potentials of PDLCs and DPCs significantly increased in response to hypoxic environment.
Conclusions.
Our findings suggest that hypoxia plays an important role in maintaining the stemness and differentiation capacity of PDLCs and DPCs.
American Psychological Association (APA)
Zhou, Yinghong& Fan, Wei& Xiao, Yin. 2014. The Effect of Hypoxia on the Stemness and Differentiation Capacity of PDLC and DPC. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-505752
Modern Language Association (MLA)
Zhou, Yinghong…[et al.]. The Effect of Hypoxia on the Stemness and Differentiation Capacity of PDLC and DPC. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-505752
American Medical Association (AMA)
Zhou, Yinghong& Fan, Wei& Xiao, Yin. The Effect of Hypoxia on the Stemness and Differentiation Capacity of PDLC and DPC. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-505752
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-505752