Evaluation of a Multiplex ELISA for Autoantibody Profiling in Patients with Autoimmune Connective Tissue Diseases

المؤلفون المشاركون

Alonso Cañizal, M. Consuelo
Jiménez Jiménez, Luis M.
Durán Parejo, Pilar
Alonso Díez, Lorena
Kumble, Sarita
Caro Pérez, Alejandro
Kumble, Krishnanand D.

المصدر

Autoimmune Diseases

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-01-16

دولة النشر

مصر

عدد الصفحات

6

التخصصات الرئيسية

الأمراض

الملخص EN

The performance of immunoassays for the detection of autoantibodies is of critical importance in the diagnosis and assessment of patients with autoimmune connective tissue diseases (ACTD).

Our objective was to compare the features of two multiplexed assays—INNO-LIA ANA and Gennova-PictArray ENA ELISA—for measurement of multiple autoantibodies and their utility as a clinical tool in ACTD diagnosis.

The antigens included SS-A/Ro (60 and 52), SSB/La, Sm, Sm/RNP, CENP-B, Jo-1, and Scl-70.

Stored sera from 85 ACTD patients and 80 controls consisting of patients with vasculitis, rheumatoid arthritis and infectious diseases, as well as healthy subjects were analyzed jointly with clinical and laboratory data.

Agreement between the two methods varied between 58 and 99% (Cohen’s kappa: 0.21–0.71) mostly for SSA and SSB.

The frequency of specific autoantibodies measured using the two methods was more variable for SSA, SSB, and RNP/Sm.

There were a higher number of ambiguous results when using INNO-LIA.

The optimized cut-off values of the Gennova-PictArray resulted in over 99% specificities in samples obtained from the control group.

Sensitivity patterns were more accurate in Gennova-PictArray than in INNO-LIA, as suggested in previously reported studies.

A third method could be applied to determine which of the two methods is more accurate.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Caro Pérez, Alejandro& Kumble, Sarita& Kumble, Krishnanand D.& Alonso Cañizal, M. Consuelo& Jiménez Jiménez, Luis M.& Alonso Díez, Lorena…[et al.]. 2014. Evaluation of a Multiplex ELISA for Autoantibody Profiling in Patients with Autoimmune Connective Tissue Diseases. Autoimmune Diseases،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-506308

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Caro Pérez, Alejandro…[et al.]. Evaluation of a Multiplex ELISA for Autoantibody Profiling in Patients with Autoimmune Connective Tissue Diseases. Autoimmune Diseases No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-506308

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Caro Pérez, Alejandro& Kumble, Sarita& Kumble, Krishnanand D.& Alonso Cañizal, M. Consuelo& Jiménez Jiménez, Luis M.& Alonso Díez, Lorena…[et al.]. Evaluation of a Multiplex ELISA for Autoantibody Profiling in Patients with Autoimmune Connective Tissue Diseases. Autoimmune Diseases. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-506308

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-506308