Evaluation of a Multiplex ELISA for Autoantibody Profiling in Patients with Autoimmune Connective Tissue Diseases
Joint Authors
Alonso Cañizal, M. Consuelo
Jiménez Jiménez, Luis M.
Durán Parejo, Pilar
Alonso Díez, Lorena
Kumble, Sarita
Caro Pérez, Alejandro
Kumble, Krishnanand D.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-16
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The performance of immunoassays for the detection of autoantibodies is of critical importance in the diagnosis and assessment of patients with autoimmune connective tissue diseases (ACTD).
Our objective was to compare the features of two multiplexed assays—INNO-LIA ANA and Gennova-PictArray ENA ELISA—for measurement of multiple autoantibodies and their utility as a clinical tool in ACTD diagnosis.
The antigens included SS-A/Ro (60 and 52), SSB/La, Sm, Sm/RNP, CENP-B, Jo-1, and Scl-70.
Stored sera from 85 ACTD patients and 80 controls consisting of patients with vasculitis, rheumatoid arthritis and infectious diseases, as well as healthy subjects were analyzed jointly with clinical and laboratory data.
Agreement between the two methods varied between 58 and 99% (Cohen’s kappa: 0.21–0.71) mostly for SSA and SSB.
The frequency of specific autoantibodies measured using the two methods was more variable for SSA, SSB, and RNP/Sm.
There were a higher number of ambiguous results when using INNO-LIA.
The optimized cut-off values of the Gennova-PictArray resulted in over 99% specificities in samples obtained from the control group.
Sensitivity patterns were more accurate in Gennova-PictArray than in INNO-LIA, as suggested in previously reported studies.
A third method could be applied to determine which of the two methods is more accurate.
American Psychological Association (APA)
Caro Pérez, Alejandro& Kumble, Sarita& Kumble, Krishnanand D.& Alonso Cañizal, M. Consuelo& Jiménez Jiménez, Luis M.& Alonso Díez, Lorena…[et al.]. 2014. Evaluation of a Multiplex ELISA for Autoantibody Profiling in Patients with Autoimmune Connective Tissue Diseases. Autoimmune Diseases،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-506308
Modern Language Association (MLA)
Caro Pérez, Alejandro…[et al.]. Evaluation of a Multiplex ELISA for Autoantibody Profiling in Patients with Autoimmune Connective Tissue Diseases. Autoimmune Diseases No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-506308
American Medical Association (AMA)
Caro Pérez, Alejandro& Kumble, Sarita& Kumble, Krishnanand D.& Alonso Cañizal, M. Consuelo& Jiménez Jiménez, Luis M.& Alonso Díez, Lorena…[et al.]. Evaluation of a Multiplex ELISA for Autoantibody Profiling in Patients with Autoimmune Connective Tissue Diseases. Autoimmune Diseases. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-506308
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506308