![](/images/graphics-bg.png)
Proof of Principle for a Real-Time Pathogen Isolation Media Diagnostic : The Use of Laser-Induced Breakdown Spectroscopy to Discriminate Bacterial Pathogens and Antimicrobial-Resistant Staphylococcus aureus Strains Grown on Blood Agar
المؤلفون المشاركون
Cremers, David A.
Multari, Rosalie A.
Dupre, Joanne M.
Gustafson, John E.
Bostian, Melissa L.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-10
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Laser-Induced Breakdown Spectroscopy (LIBS) is a rapid, in situ, diagnostic technique in which light emissions from a laser plasma formed on the sample are used for analysis allowing automated analysis results to be available in seconds to minutes.
This speed of analysis coupled with little or no sample preparation makes LIBS an attractive detection tool.
In this study, it is demonstrated that LIBS can be utilized to discriminate both the bacterial species and strains of bacterial colonies grown on blood agar.
A discrimination algorithm was created based on multivariate regression analysis of spectral data.
The algorithm was deployed on a simulated LIBS instrument system to demonstrate discrimination capability using 6 species.
Genetically altered Staphylococcus aureus strains grown on BA, including isogenic sets that differed only by the acquisition of mutations that increase fusidic acid or vancomycin resistance, were also discriminated.
The algorithm successfully identified all thirteen cultures used in this study in a time period of 2 minutes.
This work provides proof of principle for a LIBS instrumentation system that could be developed for the rapid discrimination of bacterial species and strains demonstrating relatively minor genomic alterations using data collected directly from pathogen isolation media.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Multari, Rosalie A.& Cremers, David A.& Bostian, Melissa L.& Dupre, Joanne M.& Gustafson, John E.. 2013. Proof of Principle for a Real-Time Pathogen Isolation Media Diagnostic : The Use of Laser-Induced Breakdown Spectroscopy to Discriminate Bacterial Pathogens and Antimicrobial-Resistant Staphylococcus aureus Strains Grown on Blood Agar. Journal of Pathogens،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-506440
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Multari, Rosalie A.…[et al.]. Proof of Principle for a Real-Time Pathogen Isolation Media Diagnostic : The Use of Laser-Induced Breakdown Spectroscopy to Discriminate Bacterial Pathogens and Antimicrobial-Resistant Staphylococcus aureus Strains Grown on Blood Agar. Journal of Pathogens No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-506440
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Multari, Rosalie A.& Cremers, David A.& Bostian, Melissa L.& Dupre, Joanne M.& Gustafson, John E.. Proof of Principle for a Real-Time Pathogen Isolation Media Diagnostic : The Use of Laser-Induced Breakdown Spectroscopy to Discriminate Bacterial Pathogens and Antimicrobial-Resistant Staphylococcus aureus Strains Grown on Blood Agar. Journal of Pathogens. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-506440
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-506440
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)