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Proof of Principle for a Real-Time Pathogen Isolation Media Diagnostic : The Use of Laser-Induced Breakdown Spectroscopy to Discriminate Bacterial Pathogens and Antimicrobial-Resistant Staphylococcus aureus Strains Grown on Blood Agar
Joint Authors
Cremers, David A.
Multari, Rosalie A.
Dupre, Joanne M.
Gustafson, John E.
Bostian, Melissa L.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Laser-Induced Breakdown Spectroscopy (LIBS) is a rapid, in situ, diagnostic technique in which light emissions from a laser plasma formed on the sample are used for analysis allowing automated analysis results to be available in seconds to minutes.
This speed of analysis coupled with little or no sample preparation makes LIBS an attractive detection tool.
In this study, it is demonstrated that LIBS can be utilized to discriminate both the bacterial species and strains of bacterial colonies grown on blood agar.
A discrimination algorithm was created based on multivariate regression analysis of spectral data.
The algorithm was deployed on a simulated LIBS instrument system to demonstrate discrimination capability using 6 species.
Genetically altered Staphylococcus aureus strains grown on BA, including isogenic sets that differed only by the acquisition of mutations that increase fusidic acid or vancomycin resistance, were also discriminated.
The algorithm successfully identified all thirteen cultures used in this study in a time period of 2 minutes.
This work provides proof of principle for a LIBS instrumentation system that could be developed for the rapid discrimination of bacterial species and strains demonstrating relatively minor genomic alterations using data collected directly from pathogen isolation media.
American Psychological Association (APA)
Multari, Rosalie A.& Cremers, David A.& Bostian, Melissa L.& Dupre, Joanne M.& Gustafson, John E.. 2013. Proof of Principle for a Real-Time Pathogen Isolation Media Diagnostic : The Use of Laser-Induced Breakdown Spectroscopy to Discriminate Bacterial Pathogens and Antimicrobial-Resistant Staphylococcus aureus Strains Grown on Blood Agar. Journal of Pathogens،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-506440
Modern Language Association (MLA)
Multari, Rosalie A.…[et al.]. Proof of Principle for a Real-Time Pathogen Isolation Media Diagnostic : The Use of Laser-Induced Breakdown Spectroscopy to Discriminate Bacterial Pathogens and Antimicrobial-Resistant Staphylococcus aureus Strains Grown on Blood Agar. Journal of Pathogens No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-506440
American Medical Association (AMA)
Multari, Rosalie A.& Cremers, David A.& Bostian, Melissa L.& Dupre, Joanne M.& Gustafson, John E.. Proof of Principle for a Real-Time Pathogen Isolation Media Diagnostic : The Use of Laser-Induced Breakdown Spectroscopy to Discriminate Bacterial Pathogens and Antimicrobial-Resistant Staphylococcus aureus Strains Grown on Blood Agar. Journal of Pathogens. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-506440
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-506440