Penetration of Milk-Derived Antimicrobial Peptides into Phospholipid Monolayers as Model Biomembranes

المؤلفون المشاركون

Barzyk, Wanda
Rogalska, Ewa
Więcław-Czapla, Katarzyna

المصدر

Biochemistry Research International

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-16، 16ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-12-17

دولة النشر

مصر

عدد الصفحات

16

التخصصات الرئيسية

الكيمياء

الملخص EN

Three antimicrobial peptides derived from bovine milk proteins were examined with regard to penetration into insoluble monolayers formed with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DPPG).

Effects on surface pressure (Π) and electric surface potential (ΔV) were measured, Π with a platinum Wilhelmy plate and ΔV with a vibrating plate.

The penetration measurements were performed under stationary diffusion conditions and upon the compression of the monolayers.

The two type measurements showed greatly different effects of the peptide-lipid interactions.

Results of the stationary penetration show that the peptide interactions with DPPC monolayer are weak, repulsive, and nonspecific while the interactions with DPPG monolayer are significant, attractive, and specific.

These results are in accord with the fact that antimicrobial peptides disrupt bacteria membranes (negative) while no significant effect on the host membranes (neutral) is observed.

No such discrimination was revealed from the compression isotherms.

The latter indicate that squeezing the penetrant out of the monolayer upon compression does not allow for establishing the penetration equilibrium, so the monolayer remains supersaturated with the penetrant and shows an under-equilibrium orientation within the entire compression range, practically.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Barzyk, Wanda& Rogalska, Ewa& Więcław-Czapla, Katarzyna. 2013. Penetration of Milk-Derived Antimicrobial Peptides into Phospholipid Monolayers as Model Biomembranes. Biochemistry Research International،Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-507679

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Barzyk, Wanda…[et al.]. Penetration of Milk-Derived Antimicrobial Peptides into Phospholipid Monolayers as Model Biomembranes. Biochemistry Research International No. 2013 (2013), pp.1-16.
https://search.emarefa.net/detail/BIM-507679

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Barzyk, Wanda& Rogalska, Ewa& Więcław-Czapla, Katarzyna. Penetration of Milk-Derived Antimicrobial Peptides into Phospholipid Monolayers as Model Biomembranes. Biochemistry Research International. 2013. Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-507679

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-507679