![](/images/graphics-bg.png)
Penetration of Milk-Derived Antimicrobial Peptides into Phospholipid Monolayers as Model Biomembranes
Joint Authors
Barzyk, Wanda
Rogalska, Ewa
Więcław-Czapla, Katarzyna
Source
Biochemistry Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-17
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Three antimicrobial peptides derived from bovine milk proteins were examined with regard to penetration into insoluble monolayers formed with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DPPG).
Effects on surface pressure (Π) and electric surface potential (ΔV) were measured, Π with a platinum Wilhelmy plate and ΔV with a vibrating plate.
The penetration measurements were performed under stationary diffusion conditions and upon the compression of the monolayers.
The two type measurements showed greatly different effects of the peptide-lipid interactions.
Results of the stationary penetration show that the peptide interactions with DPPC monolayer are weak, repulsive, and nonspecific while the interactions with DPPG monolayer are significant, attractive, and specific.
These results are in accord with the fact that antimicrobial peptides disrupt bacteria membranes (negative) while no significant effect on the host membranes (neutral) is observed.
No such discrimination was revealed from the compression isotherms.
The latter indicate that squeezing the penetrant out of the monolayer upon compression does not allow for establishing the penetration equilibrium, so the monolayer remains supersaturated with the penetrant and shows an under-equilibrium orientation within the entire compression range, practically.
American Psychological Association (APA)
Barzyk, Wanda& Rogalska, Ewa& Więcław-Czapla, Katarzyna. 2013. Penetration of Milk-Derived Antimicrobial Peptides into Phospholipid Monolayers as Model Biomembranes. Biochemistry Research International،Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-507679
Modern Language Association (MLA)
Barzyk, Wanda…[et al.]. Penetration of Milk-Derived Antimicrobial Peptides into Phospholipid Monolayers as Model Biomembranes. Biochemistry Research International No. 2013 (2013), pp.1-16.
https://search.emarefa.net/detail/BIM-507679
American Medical Association (AMA)
Barzyk, Wanda& Rogalska, Ewa& Więcław-Czapla, Katarzyna. Penetration of Milk-Derived Antimicrobial Peptides into Phospholipid Monolayers as Model Biomembranes. Biochemistry Research International. 2013. Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-507679
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-507679