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Simultaneous Determination of Crypto-Chlorogenic Acid, Isoquercetin, and Astragalin Contents in Moringa oleifera Leaf Extracts by TLC-Densitometric Method
المؤلفون المشاركون
Gritsanapan, Wandee
Sithisarn, Pongtip
Vongsak, Boonyadist
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-02-25
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Moringa oleifera Lamarck (Moringaceae) is used as a multipurpose medicinal plant for the treatment of various diseases.
Isoquercetin, astragalin, and crypto-chlorogenic acid have been previously found to be major active components in the leaves of this plant.
In this study, a thin-layer-chromatography (TLC-)densitometric method was developed and validated for simultaneous quantification of these major components in the 70% ethanolic extracts of M.
oleifera leaves collected from 12 locations.
The average amounts of crypto-chlorogenic acid, isoquercetin, and astragalin were found to be 0.0473, 0.0427, and 0.0534% dry weight, respectively.
The method was validated for linearity, precision, accuracy, limit of detection, limit of quantitation, and robustness.
The linearity was obtained in the range of 100–500 ng/spot with a correlation coefficient (r) over 0.9961.
Intraday and interday precisions demonstrated relative standard deviations of less than 5%.
The accuracy of the method was confirmed by determining the recovery.
The average recoveries of each component from the extracts were in the range of 98.28 to 99.65%.
Additionally, the leaves from Chiang Mai province contained the highest amounts of all active components.
The proposed TLC-densitometric method was simple, accurate, precise, and cost-effective for routine quality controlling of M.
oleifera leaf extracts.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Vongsak, Boonyadist& Sithisarn, Pongtip& Gritsanapan, Wandee. 2013. Simultaneous Determination of Crypto-Chlorogenic Acid, Isoquercetin, and Astragalin Contents in Moringa oleifera Leaf Extracts by TLC-Densitometric Method. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-507973
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Vongsak, Boonyadist…[et al.]. Simultaneous Determination of Crypto-Chlorogenic Acid, Isoquercetin, and Astragalin Contents in Moringa oleifera Leaf Extracts by TLC-Densitometric Method. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-507973
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Vongsak, Boonyadist& Sithisarn, Pongtip& Gritsanapan, Wandee. Simultaneous Determination of Crypto-Chlorogenic Acid, Isoquercetin, and Astragalin Contents in Moringa oleifera Leaf Extracts by TLC-Densitometric Method. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-507973
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-507973
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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