Simultaneous Determination of Crypto-Chlorogenic Acid, Isoquercetin, and Astragalin Contents in Moringa oleifera Leaf Extracts by TLC-Densitometric Method

Abstract EN

Moringa oleifera Lamarck (Moringaceae) is used as a multipurpose medicinal plant for the treatment of various diseases.

Isoquercetin, astragalin, and crypto-chlorogenic acid have been previously found to be major active components in the leaves of this plant.

In this study, a thin-layer-chromatography (TLC-)densitometric method was developed and validated for simultaneous quantification of these major components in the 70% ethanolic extracts of M.

oleifera leaves collected from 12 locations.

The average amounts of crypto-chlorogenic acid, isoquercetin, and astragalin were found to be 0.0473, 0.0427, and 0.0534% dry weight, respectively.

The method was validated for linearity, precision, accuracy, limit of detection, limit of quantitation, and robustness.

The linearity was obtained in the range of 100–500 ng/spot with a correlation coefficient (r) over 0.9961.

Intraday and interday precisions demonstrated relative standard deviations of less than 5%.

The accuracy of the method was confirmed by determining the recovery.

The average recoveries of each component from the extracts were in the range of 98.28 to 99.65%.

Additionally, the leaves from Chiang Mai province contained the highest amounts of all active components.

The proposed TLC-densitometric method was simple, accurate, precise, and cost-effective for routine quality controlling of M.

oleifera leaf extracts.