Purification and Characterization of BmooAi : A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation
المؤلفون المشاركون
de Morais, Nadia Cristina G.
Martins Soares, Andreimar
Mamede, Carla Cristine N.
Barbosa de Sousa, Bruna
Cortes Fonseca, Kelly
Pereira, Déborah Fernanda C.
Migliorini, Thaís M.
Ribeiro de Queiroz, Mayara
Calderon, Leonardo A.
de Oliveira, Fábio
Simões-Silva, Rodrigo
Stanziola, Leonilda
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-29
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
In this paper, we describe the purification/characterization of BmooAi, a new toxin from Bothrops moojeni that inhibits platelet aggregation.
The purification of BmooAi was carried out through three chromatographic steps (ion-exchange on a DEAE-Sephacel column, molecular exclusion on a Sephadex G-75 column, and reverse-phase HPLC chromatography on a C2/C18 column).
BmooAi was homogeneous by SDS-PAGE and shown to be a single-chain protein of 15,000 Da.
BmooAi was analysed by MALDI-TOF Spectrometry and revealed two major components with molecular masses 7824.4 and 7409.2 as well as a trace of protein with a molecular mass of 15,237.4 Da.
Sequencing of BmooAi by Edman degradation showed two amino acid sequences: IRDFDPLTNAPENTA and ETEEGAEEGTQ, which revealed no homology to any known toxin from snake venom.
BmooAi showed a rather specific inhibitory effect on platelet aggregation induced by collagen, adenosine diphosphate, or epinephrine in human platelet-rich plasma in a dose-dependent manner, whereas it had little or no effect on platelet aggregation induced by ristocetin.
The effect on platelet aggregation induced by BmooAi remained active even when heated to 100°C.
BmooAi could be of medical interest as a new tool for the development of novel therapeutic agents for the prevention and treatment of thrombotic disorders.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ribeiro de Queiroz, Mayara& Mamede, Carla Cristine N.& de Morais, Nadia Cristina G.& Cortes Fonseca, Kelly& Barbosa de Sousa, Bruna& Migliorini, Thaís M.…[et al.]. 2014. Purification and Characterization of BmooAi : A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-508278
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ribeiro de Queiroz, Mayara…[et al.]. Purification and Characterization of BmooAi : A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-508278
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ribeiro de Queiroz, Mayara& Mamede, Carla Cristine N.& de Morais, Nadia Cristina G.& Cortes Fonseca, Kelly& Barbosa de Sousa, Bruna& Migliorini, Thaís M.…[et al.]. Purification and Characterization of BmooAi : A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-508278
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-508278
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر