Purification and Characterization of BmooAi : A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation
Joint Authors
de Morais, Nadia Cristina G.
Martins Soares, Andreimar
Mamede, Carla Cristine N.
Barbosa de Sousa, Bruna
Cortes Fonseca, Kelly
Pereira, Déborah Fernanda C.
Migliorini, Thaís M.
Ribeiro de Queiroz, Mayara
Calderon, Leonardo A.
de Oliveira, Fábio
Simões-Silva, Rodrigo
Stanziola, Leonilda
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
In this paper, we describe the purification/characterization of BmooAi, a new toxin from Bothrops moojeni that inhibits platelet aggregation.
The purification of BmooAi was carried out through three chromatographic steps (ion-exchange on a DEAE-Sephacel column, molecular exclusion on a Sephadex G-75 column, and reverse-phase HPLC chromatography on a C2/C18 column).
BmooAi was homogeneous by SDS-PAGE and shown to be a single-chain protein of 15,000 Da.
BmooAi was analysed by MALDI-TOF Spectrometry and revealed two major components with molecular masses 7824.4 and 7409.2 as well as a trace of protein with a molecular mass of 15,237.4 Da.
Sequencing of BmooAi by Edman degradation showed two amino acid sequences: IRDFDPLTNAPENTA and ETEEGAEEGTQ, which revealed no homology to any known toxin from snake venom.
BmooAi showed a rather specific inhibitory effect on platelet aggregation induced by collagen, adenosine diphosphate, or epinephrine in human platelet-rich plasma in a dose-dependent manner, whereas it had little or no effect on platelet aggregation induced by ristocetin.
The effect on platelet aggregation induced by BmooAi remained active even when heated to 100°C.
BmooAi could be of medical interest as a new tool for the development of novel therapeutic agents for the prevention and treatment of thrombotic disorders.
American Psychological Association (APA)
Ribeiro de Queiroz, Mayara& Mamede, Carla Cristine N.& de Morais, Nadia Cristina G.& Cortes Fonseca, Kelly& Barbosa de Sousa, Bruna& Migliorini, Thaís M.…[et al.]. 2014. Purification and Characterization of BmooAi : A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-508278
Modern Language Association (MLA)
Ribeiro de Queiroz, Mayara…[et al.]. Purification and Characterization of BmooAi : A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-508278
American Medical Association (AMA)
Ribeiro de Queiroz, Mayara& Mamede, Carla Cristine N.& de Morais, Nadia Cristina G.& Cortes Fonseca, Kelly& Barbosa de Sousa, Bruna& Migliorini, Thaís M.…[et al.]. Purification and Characterization of BmooAi : A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-508278
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508278