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A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation
المؤلفون المشاركون
Guette, Catherine
Bourreau, Anthony
Ernoult, Emilie
Gamelin, Erick
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-11-01
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Human blood plasma contains a plethora of proteins, encompassing not only proteins that have plasma-based functionalities, but also possibly every other form of low concentrated human proteins.
As it circulates through the tissues, the plasma picks up proteins that are released from their origin due to physiological events such as tissue remodeling and cell death.
Specific disease processes or tumors are often characterized by plasma “signatures,” which may become obvious via changes in the plasma proteome profile, for example, through over expression of proteins.
However, the wide dynamic range of proteins present in plasma makes their analysis very challenging, because high-abundance proteins tend to mask those of lower abundance.
In the present study, we used a strategy combining iTRAQ as a reagent which improved the peptide ionization and peptide OFFGEL fractionation that has already been shown, in our previous research, to improve the proteome coverage of cellular extracts.
Two prefractioning methods were compared: immunodepletion and a bead-based library of combinatorial hexapeptide technology.
Our data suggested that both methods were complementary, with regard to the number of identified proteins.
iTRAQ labelling, in association with OFFGEL fractionation, allowed more than 300 different proteins to be characterized from 400 μg of plasma proteins.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ernoult, Emilie& Bourreau, Anthony& Gamelin, Erick& Guette, Catherine. 2009. A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-508831
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ernoult, Emilie…[et al.]. A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-508831
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ernoult, Emilie& Bourreau, Anthony& Gamelin, Erick& Guette, Catherine. A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation. Journal of Biomedicine and Biotechnology. 2009. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-508831
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-508831
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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