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A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation
Joint Authors
Guette, Catherine
Bourreau, Anthony
Ernoult, Emilie
Gamelin, Erick
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Human blood plasma contains a plethora of proteins, encompassing not only proteins that have plasma-based functionalities, but also possibly every other form of low concentrated human proteins.
As it circulates through the tissues, the plasma picks up proteins that are released from their origin due to physiological events such as tissue remodeling and cell death.
Specific disease processes or tumors are often characterized by plasma “signatures,” which may become obvious via changes in the plasma proteome profile, for example, through over expression of proteins.
However, the wide dynamic range of proteins present in plasma makes their analysis very challenging, because high-abundance proteins tend to mask those of lower abundance.
In the present study, we used a strategy combining iTRAQ as a reagent which improved the peptide ionization and peptide OFFGEL fractionation that has already been shown, in our previous research, to improve the proteome coverage of cellular extracts.
Two prefractioning methods were compared: immunodepletion and a bead-based library of combinatorial hexapeptide technology.
Our data suggested that both methods were complementary, with regard to the number of identified proteins.
iTRAQ labelling, in association with OFFGEL fractionation, allowed more than 300 different proteins to be characterized from 400 μg of plasma proteins.
American Psychological Association (APA)
Ernoult, Emilie& Bourreau, Anthony& Gamelin, Erick& Guette, Catherine. 2009. A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-508831
Modern Language Association (MLA)
Ernoult, Emilie…[et al.]. A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-508831
American Medical Association (AMA)
Ernoult, Emilie& Bourreau, Anthony& Gamelin, Erick& Guette, Catherine. A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation. Journal of Biomedicine and Biotechnology. 2009. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-508831
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508831