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Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans
المؤلفون المشاركون
Bustos-Jaimes, Ismael
Farrés, Amelia
Navarro-Ocaña, Arturo
Peña-Montes, Carolina
Mondragón-Tintor, María Elena
Castro-Rodríguez, José Augusto
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-05-27
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The recombinant NStcI A.
nidulans esterase was adsorbed on Accurel MP1000, where protein yield and immobilization efficiency were 42.48% and 81.94%, respectively.
Storage stability test at 4°C and RT showed 100% of residual activity after 40 days at both temperatures.
The biocatalyst retains more than 70% of its initial activity after 3 cycles of repeated use.
Biochemical properties of this new biocatalyst were obtained.
Maximum activity was achieved at pH 11 and 30°C, while the best stability was observed with the pH between 9 and 11 at 40°C.
NStcI thermostability was increased after immobilization, as it retained 47.5% of its initial activity after 1 h at 60°C, while the free enzyme under the same conditions displayed no activity.
NStcI preserved 70% of its initial activity in 100% hexane after 72 h.
Enzymatic kinetic resolution of (R,S)-1-phenylethanol was chosen as model reaction, using vinyl acetate as acyl donor.
After optimization of reaction parameters, the highest possible conversion (42%) was reached at 37°C, aw of 0.07, and 120 h of bioconversion in hexane with an enantiomeric excess of 71.7%.
NStcI has selectivity for (R)-enantiomer.
The obtained E value (31.3) is in the range considered useful to resolve enantiomeric mixtures.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Peña-Montes, Carolina& Mondragón-Tintor, María Elena& Castro-Rodríguez, José Augusto& Bustos-Jaimes, Ismael& Navarro-Ocaña, Arturo& Farrés, Amelia. 2013. Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans. Enzyme Research،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-508932
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Peña-Montes, Carolina…[et al.]. Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans. Enzyme Research No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-508932
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Peña-Montes, Carolina& Mondragón-Tintor, María Elena& Castro-Rodríguez, José Augusto& Bustos-Jaimes, Ismael& Navarro-Ocaña, Arturo& Farrés, Amelia. Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-508932
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-508932
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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