Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans
Joint Authors
Bustos-Jaimes, Ismael
Farrés, Amelia
Navarro-Ocaña, Arturo
Peña-Montes, Carolina
Mondragón-Tintor, María Elena
Castro-Rodríguez, José Augusto
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The recombinant NStcI A.
nidulans esterase was adsorbed on Accurel MP1000, where protein yield and immobilization efficiency were 42.48% and 81.94%, respectively.
Storage stability test at 4°C and RT showed 100% of residual activity after 40 days at both temperatures.
The biocatalyst retains more than 70% of its initial activity after 3 cycles of repeated use.
Biochemical properties of this new biocatalyst were obtained.
Maximum activity was achieved at pH 11 and 30°C, while the best stability was observed with the pH between 9 and 11 at 40°C.
NStcI thermostability was increased after immobilization, as it retained 47.5% of its initial activity after 1 h at 60°C, while the free enzyme under the same conditions displayed no activity.
NStcI preserved 70% of its initial activity in 100% hexane after 72 h.
Enzymatic kinetic resolution of (R,S)-1-phenylethanol was chosen as model reaction, using vinyl acetate as acyl donor.
After optimization of reaction parameters, the highest possible conversion (42%) was reached at 37°C, aw of 0.07, and 120 h of bioconversion in hexane with an enantiomeric excess of 71.7%.
NStcI has selectivity for (R)-enantiomer.
The obtained E value (31.3) is in the range considered useful to resolve enantiomeric mixtures.
American Psychological Association (APA)
Peña-Montes, Carolina& Mondragón-Tintor, María Elena& Castro-Rodríguez, José Augusto& Bustos-Jaimes, Ismael& Navarro-Ocaña, Arturo& Farrés, Amelia. 2013. Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans. Enzyme Research،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-508932
Modern Language Association (MLA)
Peña-Montes, Carolina…[et al.]. Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans. Enzyme Research No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-508932
American Medical Association (AMA)
Peña-Montes, Carolina& Mondragón-Tintor, María Elena& Castro-Rodríguez, José Augusto& Bustos-Jaimes, Ismael& Navarro-Ocaña, Arturo& Farrés, Amelia. Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-508932
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-508932