Performance Evaluation of Manual and Automated (MagNA Pure)‎ Nucleic Acid Isolation in HPV Detection and Genotyping Using Roche Linear Array HPV Test

المؤلفون المشاركون

Margari, Niki
Karakitsos, Petros
Pappas, Asimakis
Aga, Evangelia
Panayiotides, Ioannis
Chranioti, Aikaterini
Kottaridi, Christine

المصدر

Infectious Diseases in Obstetrics and Gynecology

العدد

المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-11، 11ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2011-07-09

دولة النشر

مصر

عدد الصفحات

11

التخصصات الرئيسية

الأمراض
الطب البشري

الملخص EN

Nucleic acids of human papillomavirus (HPV) isolated by manual extraction method (AmpliLute) and automated MagNA pure system were compared and evaluated with cytohistological findings in 253 women.

The concordance level between AmpliLute and MagNA was very good 93.3% (κ=0.864, P<.0001).

Overall HPVpositivity detected by AmpliLute was 57.3% (30.4% as single and 27% as multiple infections) in contrast to MagNA 54.5% (32% and 23%, resp.).

Discrepant results observed in 25 cases: 11 MagNA(−)/AmpliLute(+), 10 of which had positive histology; 5 MagNA(+)/AmpliLute(−) with negative histology; 8 MagNA(+)/AmpliLute(+): in 7 of which AmpliLute detected extra HPV genotypes and 1 MagNA(invalid)/AmpliLute(+) with positive histology.

Both methods performed well when compared against cytological (area under curve (AUC) of AmpliLute 0.712 versus 0.672 of MagNA) and histological diagnoses (AUC of AmpliLute 0.935 versus 0.877 of MagNA), with AmpliLute showing a slightly predominance over MagNA.

However, higher sensitivities, specificities, and positive/negative predictive values were obtained by AmpliLute.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Chranioti, Aikaterini& Aga, Evangelia& Margari, Niki& Kottaridi, Christine& Pappas, Asimakis& Panayiotides, Ioannis…[et al.]. 2011. Performance Evaluation of Manual and Automated (MagNA Pure) Nucleic Acid Isolation in HPV Detection and Genotyping Using Roche Linear Array HPV Test. Infectious Diseases in Obstetrics and Gynecology،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-509139

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Chranioti, Aikaterini…[et al.]. Performance Evaluation of Manual and Automated (MagNA Pure) Nucleic Acid Isolation in HPV Detection and Genotyping Using Roche Linear Array HPV Test. Infectious Diseases in Obstetrics and Gynecology No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-509139

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Chranioti, Aikaterini& Aga, Evangelia& Margari, Niki& Kottaridi, Christine& Pappas, Asimakis& Panayiotides, Ioannis…[et al.]. Performance Evaluation of Manual and Automated (MagNA Pure) Nucleic Acid Isolation in HPV Detection and Genotyping Using Roche Linear Array HPV Test. Infectious Diseases in Obstetrics and Gynecology. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-509139

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-509139