Performance Evaluation of Manual and Automated (MagNA Pure) Nucleic Acid Isolation in HPV Detection and Genotyping Using Roche Linear Array HPV Test
Joint Authors
Margari, Niki
Karakitsos, Petros
Pappas, Asimakis
Aga, Evangelia
Panayiotides, Ioannis
Chranioti, Aikaterini
Kottaridi, Christine
Source
Infectious Diseases in Obstetrics and Gynecology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Nucleic acids of human papillomavirus (HPV) isolated by manual extraction method (AmpliLute) and automated MagNA pure system were compared and evaluated with cytohistological findings in 253 women.
The concordance level between AmpliLute and MagNA was very good 93.3% (κ=0.864, P<.0001).
Overall HPVpositivity detected by AmpliLute was 57.3% (30.4% as single and 27% as multiple infections) in contrast to MagNA 54.5% (32% and 23%, resp.).
Discrepant results observed in 25 cases: 11 MagNA(−)/AmpliLute(+), 10 of which had positive histology; 5 MagNA(+)/AmpliLute(−) with negative histology; 8 MagNA(+)/AmpliLute(+): in 7 of which AmpliLute detected extra HPV genotypes and 1 MagNA(invalid)/AmpliLute(+) with positive histology.
Both methods performed well when compared against cytological (area under curve (AUC) of AmpliLute 0.712 versus 0.672 of MagNA) and histological diagnoses (AUC of AmpliLute 0.935 versus 0.877 of MagNA), with AmpliLute showing a slightly predominance over MagNA.
However, higher sensitivities, specificities, and positive/negative predictive values were obtained by AmpliLute.
American Psychological Association (APA)
Chranioti, Aikaterini& Aga, Evangelia& Margari, Niki& Kottaridi, Christine& Pappas, Asimakis& Panayiotides, Ioannis…[et al.]. 2011. Performance Evaluation of Manual and Automated (MagNA Pure) Nucleic Acid Isolation in HPV Detection and Genotyping Using Roche Linear Array HPV Test. Infectious Diseases in Obstetrics and Gynecology،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-509139
Modern Language Association (MLA)
Chranioti, Aikaterini…[et al.]. Performance Evaluation of Manual and Automated (MagNA Pure) Nucleic Acid Isolation in HPV Detection and Genotyping Using Roche Linear Array HPV Test. Infectious Diseases in Obstetrics and Gynecology No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-509139
American Medical Association (AMA)
Chranioti, Aikaterini& Aga, Evangelia& Margari, Niki& Kottaridi, Christine& Pappas, Asimakis& Panayiotides, Ioannis…[et al.]. Performance Evaluation of Manual and Automated (MagNA Pure) Nucleic Acid Isolation in HPV Detection and Genotyping Using Roche Linear Array HPV Test. Infectious Diseases in Obstetrics and Gynecology. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-509139
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509139