Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems

المؤلفون المشاركون

Zhang, Meiling
Sun, Yang
Bai, Zhiwen
Chao, Yanhong
Li, Huaming
Zhu, Wenshuai
Han, Changri
Chang, Yonghui

المصدر

Journal of Chemistry

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-6، 6ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-09-19

دولة النشر

مصر

عدد الصفحات

6

التخصصات الرئيسية

الكيمياء

الملخص EN

This paper attempts to study and optimize the affinity partitioning conditions of papain in an aqueous two-phase system (ATPS).

The effect of the amount of ionic liquids (ILs), the concentration of K2HPO4, temperature, pH, and the volume of papain solution were discussed concretely.

The optimum conditions were determined as ionic liquid was 1.4 g and K2HPO4 was 1.4 g, the extraction efficiency of papain could reach 98.33% with pH unadjusted.

The temperature and the pH of the solution are major parameters that influence the partitioning of protein in ILs-based ATPSs.

The partition of papain to the IL-rich phase was enhanced by increasing the amount of ILs, the concentration of K2HPO4, and temperature, especially at its isoelectric point.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Bai, Zhiwen& Chao, Yanhong& Zhang, Meiling& Han, Changri& Zhu, Wenshuai& Chang, Yonghui…[et al.]. 2013. Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-509725

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Bai, Zhiwen…[et al.]. Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems. Journal of Chemistry No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-509725

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Bai, Zhiwen& Chao, Yanhong& Zhang, Meiling& Han, Changri& Zhu, Wenshuai& Chang, Yonghui…[et al.]. Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems. Journal of Chemistry. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-509725

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-509725