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Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems
Joint Authors
Zhang, Meiling
Sun, Yang
Bai, Zhiwen
Chao, Yanhong
Li, Huaming
Zhu, Wenshuai
Han, Changri
Chang, Yonghui
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-19
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
This paper attempts to study and optimize the affinity partitioning conditions of papain in an aqueous two-phase system (ATPS).
The effect of the amount of ionic liquids (ILs), the concentration of K2HPO4, temperature, pH, and the volume of papain solution were discussed concretely.
The optimum conditions were determined as ionic liquid was 1.4 g and K2HPO4 was 1.4 g, the extraction efficiency of papain could reach 98.33% with pH unadjusted.
The temperature and the pH of the solution are major parameters that influence the partitioning of protein in ILs-based ATPSs.
The partition of papain to the IL-rich phase was enhanced by increasing the amount of ILs, the concentration of K2HPO4, and temperature, especially at its isoelectric point.
American Psychological Association (APA)
Bai, Zhiwen& Chao, Yanhong& Zhang, Meiling& Han, Changri& Zhu, Wenshuai& Chang, Yonghui…[et al.]. 2013. Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-509725
Modern Language Association (MLA)
Bai, Zhiwen…[et al.]. Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems. Journal of Chemistry No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-509725
American Medical Association (AMA)
Bai, Zhiwen& Chao, Yanhong& Zhang, Meiling& Han, Changri& Zhu, Wenshuai& Chang, Yonghui…[et al.]. Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems. Journal of Chemistry. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-509725
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-509725