Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells
المؤلفون المشاركون
Heather, Alison K.
Duke, Colin C.
McGrath, Kristine C. Y.
Mandadi, Sravan
Roufogalis, Basil D.
Li, Xiao-Hong
Tran, Van H.
Li, Yi-Ming
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-07-17
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Calcium signals in hepatocytes control cell growth, proliferation, and death.
Members of the transient receptor potential (TRP) cation channel superfamily are candidate calcium influx channels.
NFκB activation strictly depends on calcium influx and often induces antiapoptotic genes favouring cell survival.
Previously, we reported that S-[6]-gingerol is an efficacious agonist of the transient receptor potential cation channel subfamily V member 1 (TRPV1) in neurones.
In this study, we tested the effect of S-[6]-gingerol on HuH-7 cells using the Fluo-4 calcium assay, RT-qPCR, transient cell transfection, and luciferase measurements.
We found that S-[6]-gingerol induced a transient rise in [Ca2+]i in HuH-7 cells.
The increase in [Ca2+]i induced by S-[6]-gingerol was abolished by preincubation with EGTA and was also inhibited by the TRPV1 channel antagonist capsazepine.
Expression of TRPV1 in HuH-7 cells was confirmed by mRNA analysis as well as a test for increase of [Ca2+]i by TRPV1 agonist capsaicin and its inhibition by capsazepine.
We found that S-[6]-gingerol induced rapid NFκB activation through TRPV1 in HuH-7 cells.
Furthermore, S-[6]-gingerol-induced NFκB activation was dependent on the calcium gradient and TRPV1.
The rapid NFκB activation by S-[6]-gingerol was associated with an increase in mRNA levels of NFκB-target genes: cIAP-2, XIAP, and Bcl-2 that encode antiapoptotic proteins.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Xiao-Hong& McGrath, Kristine C. Y.& Tran, Van H.& Li, Yi-Ming& Mandadi, Sravan& Duke, Colin C.…[et al.]. 2013. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-510889
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Xiao-Hong…[et al.]. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-510889
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Xiao-Hong& McGrath, Kristine C. Y.& Tran, Van H.& Li, Yi-Ming& Mandadi, Sravan& Duke, Colin C.…[et al.]. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-510889
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-510889
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر