Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells
Joint Authors
Heather, Alison K.
Duke, Colin C.
McGrath, Kristine C. Y.
Mandadi, Sravan
Roufogalis, Basil D.
Li, Xiao-Hong
Tran, Van H.
Li, Yi-Ming
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Calcium signals in hepatocytes control cell growth, proliferation, and death.
Members of the transient receptor potential (TRP) cation channel superfamily are candidate calcium influx channels.
NFκB activation strictly depends on calcium influx and often induces antiapoptotic genes favouring cell survival.
Previously, we reported that S-[6]-gingerol is an efficacious agonist of the transient receptor potential cation channel subfamily V member 1 (TRPV1) in neurones.
In this study, we tested the effect of S-[6]-gingerol on HuH-7 cells using the Fluo-4 calcium assay, RT-qPCR, transient cell transfection, and luciferase measurements.
We found that S-[6]-gingerol induced a transient rise in [Ca2+]i in HuH-7 cells.
The increase in [Ca2+]i induced by S-[6]-gingerol was abolished by preincubation with EGTA and was also inhibited by the TRPV1 channel antagonist capsazepine.
Expression of TRPV1 in HuH-7 cells was confirmed by mRNA analysis as well as a test for increase of [Ca2+]i by TRPV1 agonist capsaicin and its inhibition by capsazepine.
We found that S-[6]-gingerol induced rapid NFκB activation through TRPV1 in HuH-7 cells.
Furthermore, S-[6]-gingerol-induced NFκB activation was dependent on the calcium gradient and TRPV1.
The rapid NFκB activation by S-[6]-gingerol was associated with an increase in mRNA levels of NFκB-target genes: cIAP-2, XIAP, and Bcl-2 that encode antiapoptotic proteins.
American Psychological Association (APA)
Li, Xiao-Hong& McGrath, Kristine C. Y.& Tran, Van H.& Li, Yi-Ming& Mandadi, Sravan& Duke, Colin C.…[et al.]. 2013. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-510889
Modern Language Association (MLA)
Li, Xiao-Hong…[et al.]. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-510889
American Medical Association (AMA)
Li, Xiao-Hong& McGrath, Kristine C. Y.& Tran, Van H.& Li, Yi-Ming& Mandadi, Sravan& Duke, Colin C.…[et al.]. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-510889
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-510889