Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells

Joint Authors

Heather, Alison K.
Duke, Colin C.
McGrath, Kristine C. Y.
Mandadi, Sravan
Roufogalis, Basil D.
Li, Xiao-Hong
Tran, Van H.
Li, Yi-Ming

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-07-17

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Medicine

Abstract EN

Calcium signals in hepatocytes control cell growth, proliferation, and death.

Members of the transient receptor potential (TRP) cation channel superfamily are candidate calcium influx channels.

NFκB activation strictly depends on calcium influx and often induces antiapoptotic genes favouring cell survival.

Previously, we reported that S-[6]-gingerol is an efficacious agonist of the transient receptor potential cation channel subfamily V member 1 (TRPV1) in neurones.

In this study, we tested the effect of S-[6]-gingerol on HuH-7 cells using the Fluo-4 calcium assay, RT-qPCR, transient cell transfection, and luciferase measurements.

We found that S-[6]-gingerol induced a transient rise in [Ca2+]i in HuH-7 cells.

The increase in [Ca2+]i induced by S-[6]-gingerol was abolished by preincubation with EGTA and was also inhibited by the TRPV1 channel antagonist capsazepine.

Expression of TRPV1 in HuH-7 cells was confirmed by mRNA analysis as well as a test for increase of [Ca2+]i by TRPV1 agonist capsaicin and its inhibition by capsazepine.

We found that S-[6]-gingerol induced rapid NFκB activation through TRPV1 in HuH-7 cells.

Furthermore, S-[6]-gingerol-induced NFκB activation was dependent on the calcium gradient and TRPV1.

The rapid NFκB activation by S-[6]-gingerol was associated with an increase in mRNA levels of NFκB-target genes: cIAP-2, XIAP, and Bcl-2 that encode antiapoptotic proteins.

American Psychological Association (APA)

Li, Xiao-Hong& McGrath, Kristine C. Y.& Tran, Van H.& Li, Yi-Ming& Mandadi, Sravan& Duke, Colin C.…[et al.]. 2013. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-510889

Modern Language Association (MLA)

Li, Xiao-Hong…[et al.]. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-510889

American Medical Association (AMA)

Li, Xiao-Hong& McGrath, Kristine C. Y.& Tran, Van H.& Li, Yi-Ming& Mandadi, Sravan& Duke, Colin C.…[et al.]. Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-510889

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-510889