Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid : Comparative Characterization on the Nanoscale

المؤلفون المشاركون

Cillessen, Lisa
Wanekaya, Adam
Barber, Stephanie
Black, John
Ghosh, Kartik
Fisher, Michael
Reynolds, Chris
Schaeffer, Ashley
DeLong, Robert K.
Flores, Kristin J.
Severs, Tiffany

المصدر

Journal of Nanotechnology

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2011-10-19

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

العلوم الهندسية و تكنولوجيا المعلومات
الكيمياء

الملخص EN

DNA and RNA micro- and nanoparticles are increasingly being used for gene and siRNA drug delivery and a variety of other applications in bionanotechnology.

On the nanoscale, these entities represent unique challenges from a physicochemical characterization perspective.

Here, nucleic acid conjugates with protamine and gold nanoparticles (GNP) were characterized comparatively in the nanorange of concentration by UV/Vis NanoDrop spectroscopy, fluorimetry, and gel electrophoresis.

Given the intense interest in splice-site switching oligomers (SSOs), we utilized a human tumor cell culture system (HeLa pLuc-705), in which SSO-directed splicing repair upregulates luciferase expression, in order to investigate bioactivity of the bionanoconjugates.

Process parameters important for bioactivity were investigated, and the bimolecular nanoconjugates were confirmed by shifts in the dynamic laser light scatter (DLLS), UV/Vis spectrum, gel electrophoresis, or sedimentation pattern.

The data presented herein may be useful in the future development of pharmaceutical and biotechnology formulations, processes, and analyses concerning protein, DNA, or RNA bionanoconjugates.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

DeLong, Robert K.& Cillessen, Lisa& Reynolds, Chris& Wanekaya, Adam& Severs, Tiffany& Ghosh, Kartik…[et al.]. 2011. Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid : Comparative Characterization on the Nanoscale. Journal of Nanotechnology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-511165

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

DeLong, Robert K.…[et al.]. Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid : Comparative Characterization on the Nanoscale. Journal of Nanotechnology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-511165

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

DeLong, Robert K.& Cillessen, Lisa& Reynolds, Chris& Wanekaya, Adam& Severs, Tiffany& Ghosh, Kartik…[et al.]. Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid : Comparative Characterization on the Nanoscale. Journal of Nanotechnology. 2011. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-511165

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-511165