Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid : Comparative Characterization on the Nanoscale
Joint Authors
Cillessen, Lisa
Wanekaya, Adam
Barber, Stephanie
Black, John
Ghosh, Kartik
Fisher, Michael
Reynolds, Chris
Schaeffer, Ashley
DeLong, Robert K.
Flores, Kristin J.
Severs, Tiffany
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Engineering Sciences and Information Technology
Chemistry
Abstract EN
DNA and RNA micro- and nanoparticles are increasingly being used for gene and siRNA drug delivery and a variety of other applications in bionanotechnology.
On the nanoscale, these entities represent unique challenges from a physicochemical characterization perspective.
Here, nucleic acid conjugates with protamine and gold nanoparticles (GNP) were characterized comparatively in the nanorange of concentration by UV/Vis NanoDrop spectroscopy, fluorimetry, and gel electrophoresis.
Given the intense interest in splice-site switching oligomers (SSOs), we utilized a human tumor cell culture system (HeLa pLuc-705), in which SSO-directed splicing repair upregulates luciferase expression, in order to investigate bioactivity of the bionanoconjugates.
Process parameters important for bioactivity were investigated, and the bimolecular nanoconjugates were confirmed by shifts in the dynamic laser light scatter (DLLS), UV/Vis spectrum, gel electrophoresis, or sedimentation pattern.
The data presented herein may be useful in the future development of pharmaceutical and biotechnology formulations, processes, and analyses concerning protein, DNA, or RNA bionanoconjugates.
American Psychological Association (APA)
DeLong, Robert K.& Cillessen, Lisa& Reynolds, Chris& Wanekaya, Adam& Severs, Tiffany& Ghosh, Kartik…[et al.]. 2011. Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid : Comparative Characterization on the Nanoscale. Journal of Nanotechnology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-511165
Modern Language Association (MLA)
DeLong, Robert K.…[et al.]. Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid : Comparative Characterization on the Nanoscale. Journal of Nanotechnology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-511165
American Medical Association (AMA)
DeLong, Robert K.& Cillessen, Lisa& Reynolds, Chris& Wanekaya, Adam& Severs, Tiffany& Ghosh, Kartik…[et al.]. Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid : Comparative Characterization on the Nanoscale. Journal of Nanotechnology. 2011. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-511165
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511165