Metabolism of Genipin in Rat and Identification of Metabolites by Using Ultraperformance Liquid ChromatographyQuadrupole Time-of-Flight Tandem Mass Spectrometry
المؤلفون المشاركون
Zhang, Li-Ying
Zhang, Tong
Chen, Yi
Cai, Zhen-Zhen
Yang, Li
Zhang, Yong
Hou, Jian-Wei
Ding, Yue
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-03-19
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
The in vivo and in vitro metabolism of genipin was systematically investigated in the present study.
Urine, plasma, feces, and bile were collected from rats after oral administration of genipin at a dose of 50 mg/kg body weight.
A rapid and sensitive method using ultraperformance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-Q/TOF MS) was developed for analysis of metabolic profile of genipin in rat biological samples (urine, plasma, feces, and bile).
A total of ten metabolites were detected and identified by comparing their fragmentation patterns with that of genipin using MetaboLynx software tools.
On the basis of the chromatographic peak area, the sulfated and glucuronidated conjugates of genipin were identified as major metabolites.
And the existence of major metabolites G1 and G2 was confirmed by the in vitro enzymatic study further.
Then, metabolite G1 was isolated from rat bile by semipreparative HPLC.
Its structure was unambiguously identified as genipin-1-o-glucuronic acid by comparison of its UV, IR, ESI-MS, 1H-NMR, and 13C-NMR spectra with conference.
In general, genipin was a very active compound that would transform immediately, and the parent form of genipin could not be observed in rats biological samples.
The biotransformation pathways of genipin involved demethylated, ring-opened, cysteine-conjugated, hydroformylated, glucuronidated, and sulfated transformations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ding, Yue& Hou, Jian-Wei& Zhang, Yong& Zhang, Li-Ying& Zhang, Tong& Chen, Yi…[et al.]. 2013. Metabolism of Genipin in Rat and Identification of Metabolites by Using Ultraperformance Liquid ChromatographyQuadrupole Time-of-Flight Tandem Mass Spectrometry. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-511300
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ding, Yue…[et al.]. Metabolism of Genipin in Rat and Identification of Metabolites by Using Ultraperformance Liquid ChromatographyQuadrupole Time-of-Flight Tandem Mass Spectrometry. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-511300
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ding, Yue& Hou, Jian-Wei& Zhang, Yong& Zhang, Li-Ying& Zhang, Tong& Chen, Yi…[et al.]. Metabolism of Genipin in Rat and Identification of Metabolites by Using Ultraperformance Liquid ChromatographyQuadrupole Time-of-Flight Tandem Mass Spectrometry. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-511300
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-511300
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر