Metabolism of Genipin in Rat and Identification of Metabolites by Using Ultraperformance Liquid ChromatographyQuadrupole Time-of-Flight Tandem Mass Spectrometry
Joint Authors
Zhang, Li-Ying
Zhang, Tong
Chen, Yi
Cai, Zhen-Zhen
Yang, Li
Zhang, Yong
Hou, Jian-Wei
Ding, Yue
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-19
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The in vivo and in vitro metabolism of genipin was systematically investigated in the present study.
Urine, plasma, feces, and bile were collected from rats after oral administration of genipin at a dose of 50 mg/kg body weight.
A rapid and sensitive method using ultraperformance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-Q/TOF MS) was developed for analysis of metabolic profile of genipin in rat biological samples (urine, plasma, feces, and bile).
A total of ten metabolites were detected and identified by comparing their fragmentation patterns with that of genipin using MetaboLynx software tools.
On the basis of the chromatographic peak area, the sulfated and glucuronidated conjugates of genipin were identified as major metabolites.
And the existence of major metabolites G1 and G2 was confirmed by the in vitro enzymatic study further.
Then, metabolite G1 was isolated from rat bile by semipreparative HPLC.
Its structure was unambiguously identified as genipin-1-o-glucuronic acid by comparison of its UV, IR, ESI-MS, 1H-NMR, and 13C-NMR spectra with conference.
In general, genipin was a very active compound that would transform immediately, and the parent form of genipin could not be observed in rats biological samples.
The biotransformation pathways of genipin involved demethylated, ring-opened, cysteine-conjugated, hydroformylated, glucuronidated, and sulfated transformations.
American Psychological Association (APA)
Ding, Yue& Hou, Jian-Wei& Zhang, Yong& Zhang, Li-Ying& Zhang, Tong& Chen, Yi…[et al.]. 2013. Metabolism of Genipin in Rat and Identification of Metabolites by Using Ultraperformance Liquid ChromatographyQuadrupole Time-of-Flight Tandem Mass Spectrometry. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-511300
Modern Language Association (MLA)
Ding, Yue…[et al.]. Metabolism of Genipin in Rat and Identification of Metabolites by Using Ultraperformance Liquid ChromatographyQuadrupole Time-of-Flight Tandem Mass Spectrometry. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-511300
American Medical Association (AMA)
Ding, Yue& Hou, Jian-Wei& Zhang, Yong& Zhang, Li-Ying& Zhang, Tong& Chen, Yi…[et al.]. Metabolism of Genipin in Rat and Identification of Metabolites by Using Ultraperformance Liquid ChromatographyQuadrupole Time-of-Flight Tandem Mass Spectrometry. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-511300
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-511300