Carbapenem Resistance among Enterobacter Species in a Tertiary Care Hospital in Central India
المؤلفون المشاركون
Praharaj, Ashok Kumar
Kumar, Mahadevan
Grover, Naveen
Khajuria, Atul
المصدر
Chemotherapy Research and Practice
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-10
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Objective.
To detect genes encoding carbapenem resistance among Enterobacter species in a tertiary care hospital in central India.
Methods.
Bacterial identification of Enterobacter spp.
isolates from various clinical specimens in patients admitted to intensive care units was performed by routine conventional microbial culture and biochemical tests using standard recommended techniques.
Antibiotic sensitivity test was performed by standard Kirby Bauer disc diffusion technique.
PCR amplification and automated sequencing was carried out.
Transfer of resistance genes was determined by conjugation.
Results.
A total of 70/130 (53.84%) isolates of Enterobacter spp.
were found to exhibit reduced susceptibility to imipenem (diameter of zones of inhibition ≤13 mm) by disc diffusion method.
Among 70 isolates tested, 48 (68.57%) isolates showed MIC values for imipenem and meropenem ranging from 32 to 64 mg/L as per CLSI breakpoints.
All of these 70 isolates were found susceptible to colistin in vitro as per MIC breakpoints (<0.5 mg/L).
PCR carried out on these 48 MBL (IP/IPI) E-test positive isolates (12 Enterobacter aerogenes, 31 Enterobacter cloacae, and 05 Enterobacter cloacae complex) was validated by sequencing for beta-lactam resistance genes and result was interpreted accordingly.
Conclusion.
The study showed MBL production as an important mechanism in carbapenem resistance in Enterobacter spp.
and interspecies transfer of these genes through plasmids suggesting early detection by molecular methods.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Khajuria, Atul& Praharaj, Ashok Kumar& Kumar, Mahadevan& Grover, Naveen. 2014. Carbapenem Resistance among Enterobacter Species in a Tertiary Care Hospital in Central India. Chemotherapy Research and Practice،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-512577
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Khajuria, Atul…[et al.]. Carbapenem Resistance among Enterobacter Species in a Tertiary Care Hospital in Central India. Chemotherapy Research and Practice No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-512577
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Khajuria, Atul& Praharaj, Ashok Kumar& Kumar, Mahadevan& Grover, Naveen. Carbapenem Resistance among Enterobacter Species in a Tertiary Care Hospital in Central India. Chemotherapy Research and Practice. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-512577
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-512577
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر