Carbapenem Resistance among Enterobacter Species in a Tertiary Care Hospital in Central India
Joint Authors
Praharaj, Ashok Kumar
Kumar, Mahadevan
Grover, Naveen
Khajuria, Atul
Source
Chemotherapy Research and Practice
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Objective.
To detect genes encoding carbapenem resistance among Enterobacter species in a tertiary care hospital in central India.
Methods.
Bacterial identification of Enterobacter spp.
isolates from various clinical specimens in patients admitted to intensive care units was performed by routine conventional microbial culture and biochemical tests using standard recommended techniques.
Antibiotic sensitivity test was performed by standard Kirby Bauer disc diffusion technique.
PCR amplification and automated sequencing was carried out.
Transfer of resistance genes was determined by conjugation.
Results.
A total of 70/130 (53.84%) isolates of Enterobacter spp.
were found to exhibit reduced susceptibility to imipenem (diameter of zones of inhibition ≤13 mm) by disc diffusion method.
Among 70 isolates tested, 48 (68.57%) isolates showed MIC values for imipenem and meropenem ranging from 32 to 64 mg/L as per CLSI breakpoints.
All of these 70 isolates were found susceptible to colistin in vitro as per MIC breakpoints (<0.5 mg/L).
PCR carried out on these 48 MBL (IP/IPI) E-test positive isolates (12 Enterobacter aerogenes, 31 Enterobacter cloacae, and 05 Enterobacter cloacae complex) was validated by sequencing for beta-lactam resistance genes and result was interpreted accordingly.
Conclusion.
The study showed MBL production as an important mechanism in carbapenem resistance in Enterobacter spp.
and interspecies transfer of these genes through plasmids suggesting early detection by molecular methods.
American Psychological Association (APA)
Khajuria, Atul& Praharaj, Ashok Kumar& Kumar, Mahadevan& Grover, Naveen. 2014. Carbapenem Resistance among Enterobacter Species in a Tertiary Care Hospital in Central India. Chemotherapy Research and Practice،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-512577
Modern Language Association (MLA)
Khajuria, Atul…[et al.]. Carbapenem Resistance among Enterobacter Species in a Tertiary Care Hospital in Central India. Chemotherapy Research and Practice No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-512577
American Medical Association (AMA)
Khajuria, Atul& Praharaj, Ashok Kumar& Kumar, Mahadevan& Grover, Naveen. Carbapenem Resistance among Enterobacter Species in a Tertiary Care Hospital in Central India. Chemotherapy Research and Practice. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-512577
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-512577