Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B
المؤلفون المشاركون
Sarzi-Puttini, Piercarlo
Benucci, M.
Atzeni, Fabiola
Seaman, A.
Meacci, F.
Manfredi, Mariangela
Bentow, C.
Olivito, B.
Infantino, M.
Mahler, Michael
المصدر
Clinical and Developmental Immunology
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-27
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objective.
We aimed to compare a chemiluminescent immunoassay (CIA, QUANTA Flash) on BIO-FLASH with a multiplex flow immunoassay (MFI) on BioPlex 2200 for the detection of antibodies to Ro60, Ro52, and SS-B.
Methods.
The study included 241 samples, from patients suffering from systemic autoimmune diseases (n=108) as well as disease controls (n=133).
All samples were tested for anti-Ro52, anti-Ro60, and anti-SS-B (La) antibodies on QUANTA Flash (INOVA Diagnostics, San Diego, USA) and BioPlex 2200 (Bio-Rad Laboratories Inc., Hercules, USA).
Discrepant samples were tested by two independent methods: BlueDot/ANA and QUANTRIX Microarray (both D-tek, Belgium).
Results.
The overall qualitative agreements were 95.4% (95% confidence interval, CI 92.0–97.7%) for anti-Ro52, 98.8% (95% CI 96.4–99.7%) for anti-Ro60, and 91.7% (95% CI 87.5–94.9%) for anti-SS-B antibodies.
There were 34 discrepant samples among all assays (20 anti-SS-B, 11 anti-Ro52, 3 anti-Ro60).
30/33 of retested samples (by D-tek dot blot) agreed with the QUANTA Flash results.
Similar findings were obtained with QUANTRIX Microarray kit.
Conclusion.
QUANTA Flash and BioPlex 2200 show good qualitative agreement.
The clinical performances were similar for anti-Ro52 and anti-Ro60 autoantibodies while differences were observed for anti-SS-B (La) antibodies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Infantino, M.& Bentow, C.& Seaman, A.& Benucci, M.& Atzeni, Fabiola& Sarzi-Puttini, Piercarlo…[et al.]. 2013. Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-513022
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Infantino, M.…[et al.]. Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B. Clinical and Developmental Immunology No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-513022
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Infantino, M.& Bentow, C.& Seaman, A.& Benucci, M.& Atzeni, Fabiola& Sarzi-Puttini, Piercarlo…[et al.]. Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-513022
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-513022
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر