Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B
Joint Authors
Sarzi-Puttini, Piercarlo
Benucci, M.
Atzeni, Fabiola
Seaman, A.
Meacci, F.
Manfredi, Mariangela
Bentow, C.
Olivito, B.
Infantino, M.
Mahler, Michael
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
We aimed to compare a chemiluminescent immunoassay (CIA, QUANTA Flash) on BIO-FLASH with a multiplex flow immunoassay (MFI) on BioPlex 2200 for the detection of antibodies to Ro60, Ro52, and SS-B.
Methods.
The study included 241 samples, from patients suffering from systemic autoimmune diseases (n=108) as well as disease controls (n=133).
All samples were tested for anti-Ro52, anti-Ro60, and anti-SS-B (La) antibodies on QUANTA Flash (INOVA Diagnostics, San Diego, USA) and BioPlex 2200 (Bio-Rad Laboratories Inc., Hercules, USA).
Discrepant samples were tested by two independent methods: BlueDot/ANA and QUANTRIX Microarray (both D-tek, Belgium).
Results.
The overall qualitative agreements were 95.4% (95% confidence interval, CI 92.0–97.7%) for anti-Ro52, 98.8% (95% CI 96.4–99.7%) for anti-Ro60, and 91.7% (95% CI 87.5–94.9%) for anti-SS-B antibodies.
There were 34 discrepant samples among all assays (20 anti-SS-B, 11 anti-Ro52, 3 anti-Ro60).
30/33 of retested samples (by D-tek dot blot) agreed with the QUANTA Flash results.
Similar findings were obtained with QUANTRIX Microarray kit.
Conclusion.
QUANTA Flash and BioPlex 2200 show good qualitative agreement.
The clinical performances were similar for anti-Ro52 and anti-Ro60 autoantibodies while differences were observed for anti-SS-B (La) antibodies.
American Psychological Association (APA)
Infantino, M.& Bentow, C.& Seaman, A.& Benucci, M.& Atzeni, Fabiola& Sarzi-Puttini, Piercarlo…[et al.]. 2013. Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-513022
Modern Language Association (MLA)
Infantino, M.…[et al.]. Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B. Clinical and Developmental Immunology No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-513022
American Medical Association (AMA)
Infantino, M.& Bentow, C.& Seaman, A.& Benucci, M.& Atzeni, Fabiola& Sarzi-Puttini, Piercarlo…[et al.]. Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-513022
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-513022