Phenotypic and genotypic studies on Escherichia coli strains isolated from food products of animal origin

الملخص EN

Foodstuffs of animal origin may present hazards, due to bacterial contamination.

This study was conducted to determine Escherichia coli in some types of animal source foods (raw milk, raw beef meat and raw poultry meat).

Bacteriological examination of total 246 raw food samples of animal origin, raw milk, (105) ; beef meat (31) and poultry meat (110) were collected from different localities in Ismailia City showed that 64 / 246 (26.01 %) of samples were infected with E.

coli.

Serological identification of (10) E.

coli strains revealed that they were belonged to O111 polyvalent 1 ; O44 polyvalent 2 (2 strains for each) ; O78 polyvalent 4 ; O146 polyvalent 2 ; O26 polyvalent 1 ; O119 polyvalent 1 ; O86 polyvalent 1 and O18 polyvalent 3 (1 strain for each).

E.

coli strains showed high susceptible rate to Ciprofloxacin (CIP), (100 %) and high resistance (100 %) to Ampicillin (AMP), Metronidazole (MTZ), Amoxicillin (AML), and Vancomycin (VAS).

Four strains of the isolated E.

coli were submitted to molecular studies for detection of the eaeA (protein intimin), iutA (encoding aerobactin) and iss (increased serum survival protein) genes, by using PCR technique.