Evaluating the effect of selected medicinal plant extracts and their synergistic effect with metronidazole against entamoeba histolytica

العناوين الأخرى

تقييم أثر بعض النباتات الطبية و مدى تأزرها مع المترونايدازول على مسبب الزحار الاميبى (انتاميبا هستوليتكا)‎

مقدم أطروحة جامعية

Dardunah, Zuhayr Wadi Yusuf

مشرف أطروحة جامعية

al-Hindi, Adnan Ibrahim

أعضاء اللجنة

al-Sharif, Fadl Akram
al-Rayyan, Abd al-Nasir Ismail

الجامعة

الجامعة الإسلامية

الكلية

كلية العلوم

القسم الأكاديمي

قسم العلوم الحياتية

دولة الجامعة

فلسطين (قطاع غزة)

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2014

الملخص الإنجليزي

Introduction: Intestinal infection with Entamoeba histolytica is an important cause of diarrhoea world-wide especially where sanitation conditions are poor.

Medicinal plants have played a significant role in various traditional systems of medications including intestinal infections caused by E.

histolytica.

Objectives: To investigate the antiamoebic activity of pomegranate, castor and thyme aqueous and alcoholic extracts, in addition to thyme oil and pomergnate juice against E.

histolytica and to determine the synergistic effect of these plant extracts with the antibiotic metronidazole.

Methods: Three medicinal plants pomegranate (Punica granatum), castor (Ricinus communis L) and thyme (Thymus vulgaris L) were used in this study.

Some of these plants were bought from fruit's market in Gaza and some were collected from some regions in Gaza; each plant was dried then extracted according to standard extraction method using Soxhlet apparatus.

E.

histolytica was cultured in a modified diphasic liver infusion agar medium in vitro conditions to study the effect of these plant extracts on E.

histolytica and their synergestic effect with the antibiotic metronidazole.

The type of E.

histolytica was confirmed using PCR.

Results: Successful cultures of E.

histolytica were obtained.

All the plant materials used in this study possessed antiamoebic activity in vitro with different levels except the aqueous extract of R.

communis leaves which also was the only one didn‟t show synergistic effect with metronidazole at tested concentrations.

Alcoholic extract of P.

granatum pulp had the strongest antiamoebic activity with MIC of < 5 mg/ml while the aqueous extract of T.

vulgaris leaves had the weakest antiamoebic activity with a MIC of < 20 mg/ml

التخصصات الرئيسية

الأحياء

الموضوعات

• النباتات الطبية

عدد الصفحات

104

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-533885